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A9576

Sigma-Aldrich

Bovine Serum Albumin solution

30% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

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Synonym(s):
Albumin solution from bovine serum, BSA solution
CAS Number:
MDL number:
NACRES:
NA.75

biological source

bovine

sterility

sterile-filtered

product line

BioXtra

Assay

28-32% total protein basis (UV)

form

liquid

origin

USA origin

concentration

in phosphate buffer (DPBS)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

6.5-7.5

foreign activity

Mycoplasma Test by Barile on Raw material, none detected

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
A7979A1595A0336
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

aseptically filled

assay

28-32% total protein basis (UV)

assay

33-37% total protein basis (UV)

assay

90-120 mg/mL protein basis (biuret)

assay

28-32% protein basis (biuret)

form

liquid

form

liquid

form

solution

form

solution

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

concentration

in phosphate buffer (DPBS)

concentration

35% in DPBS

concentration

10% in DPBS

concentration

28-32% (biuret), 30% in saline

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

BSA 30% solution is a convenient form for direct supplementation of serum-free media. Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Preparation Note

Prepared from A9647 in DPBS (D5652).

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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