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A9883

ADA

≥98% (titration)

Synonym(s):

N-(2-Acetamido)iminodiacetic acid, N-(Carbamoylmethyl)iminodiacetic acid

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About This Item

Linear Formula:
H2NCOCH2N(CH2CO2H)2
CAS Number:
26239-55-4
Molecular Weight:
190.15
Beilstein/REAXYS Number:
1787181
EC Number:
247-530-0
MDL number:
MFCD00008031
UNSPSC Code:
12161700
PubChem Substance ID:
24891404
NACRES:
NA.25

Key Documents

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Quality Level

400

assay

≥98% (titration)

form

powder

pH

1.5-3.0

useful pH range

6.0-7.2

pKa (25 °C)

6.6

mp

219 °C (dec.) (lit.)

solubility

1 M NaOH: 160 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

NC(=O)CN(CC(O)=O)CC(O)=O

InChI

1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)

InChI key

QZTKDVCDBIDYMD-UHFFFAOYSA-N

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1 of 4

This Item
A50430030710102105001
ADA ≥98% (titration)

Sigma-Aldrich

A9883

ADA

Adenosine Deaminase from bovine spleen Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

Sigma-Aldrich

A5043

Adenosine Deaminase from bovine spleen

ADA BioUltra, ≥99.0% (T)

Sigma-Aldrich

00307

ADA

Adenosine Deaminase (ADA) from calf intestine

Roche

10102105001

Adenosine Deaminase (ADA)

assay

≥98% (titration)

assay

-

assay

≥99.0% (T)

assay

-

Quality Level

400

Quality Level

200

Quality Level

200

Quality Level

100

form

powder

form

buffered aqueous glycerol solution

form

powder or crystals

form

solution

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

solubility

1 M NaOH: 160 mg/mL, clear, colorless

solubility

-

solubility

H2O: 0.05 M, clear, colorless

solubility

-

General description

ADA is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the Good buffers developed in the 1960′s to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties.[1] The useful range of ADA buffer in aqueous solution is 6.0 - 7.2.

Application

Biological buffer component with sodium hydroxide and sodium chloride (pH 5.67-7.57, useful pH range 6.4-7.4). Used to prepare immobilized pH gradients.

Preparation Note

This product is soluble in 1 M NaOH (160mg/mL), yielding a clear, colorless solution.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000497683
0000497681
0000497681
0000497683
0000467032

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Hydrogen ion buffers for biological research
Good NE, et al.
Biochemistry, 5( (2), 467-477 (1966)
Harold Bays et al.
The Journal of clinical endocrinology and metabolism, 100(8), 3140-3148 (2015-06-02)
Despite current standard of care, many patients at high risk of cardiovascular disease (CVD) still have elevated low-density lipoprotein cholesterol (LDL-C) levels. Alirocumab is a fully human monoclonal antibody inhibitor of proprotein convertase subtilisin/kexin type 9. The objective of the
Johannan F Brandse et al.
Journal of Crohn's & colitis, 9(11), 973-981 (2015-06-28)
High serum concentrations of infliximab [IFX] and adalimumab [ADA] may be associated with adverse effects in patients with inflammatory bowel disease [IBD]. We aimed to investigate whether high anti-tumour necrosis factor [TNF] trough levels [TLs] were associated with toxicity and
Gulen Yesilbag Tonga et al.
Nature chemistry, 7(7), 597-603 (2015-06-24)
Bioorthogonal catalysis broadens the functional possibilities of intracellular chemistry. Effective delivery and regulation of synthetic catalytic systems in cells are challenging due to the complex intracellular environment and catalyst instability. Here, we report the fabrication of protein-sized bioorthogonal nanozymes through
Eoin Gillespie et al.
Journal of separation science, 32(15-16), 2659-2667 (2009-07-09)
The application of scanning capacitively coupled contactless conductivity detection (SC(4)D) for the determination of pH dependant behaviour of two aminopolycarboxylates immobilised onto the surface of a monolithic capillary column is described. The use of SC(4)D to visualise changes in conductivity
View All Related Papers

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