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A9919

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:45,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A0418A3687A9169
biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

direct ELISA: 1:45,000, western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

direct ELISA: 1:20,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:100,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

Binds all rabbit Igs.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family. It is widely expressed and consists of a gamma (γ) heavy chain in the constant (C) region. IgG is a monomer with a molecular weight of about 150 kDa. The structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of the antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the CH2 and CH3 domains of the antibody molecule.

Immunogen

purified rabbit IgG

Application

Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for immunohistochemistry and western blot applications. The antibody can also be used for direct ELISA at 1:45,000.
Lysates of human fibroblasts and Hela cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:30000 dilution.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide as preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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