A9968

N-Acetyl-Ile-Glu-Thr-Asp-p-nitroanilide

≥95%

• Synonym(s): Ac-IETD-pNA
• Empirical Formula (Hill Notation): C₂₇H₃₈N₆O₁₂
• Molecular Weight: 638.62
• MDL number: MFCD01318840
• UNSPSC Code: 12352209
• PubChem Substance ID: 329771065
• NACRES: NA.32

Properties

• Quality Level: 200
• assay: ≥95%
• form: powder
• storage temp.: −20°C
• SMILES string: CC[C@H](C)[C@H](NC(C)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C27H38N6O12/c1-5-13(2)22(28-15(4)35)26(42)30-18(10-11-20(36)37)24(40)32-23(14(3)34)27(43)31-19(12-21(38)39)25(41)29-16-6-8-17(9-7-16)33(44)45/h6-9,13-14,18-19,22-23,34H,5,10-12H2,1-4H3,(H,28,35)(H,29,41)(H,30,42)(H,31,43)(H,32,40)(H,36,37)(H,38,39)/t13-,14+,18-,19-,22-,23-/m0/s1
• InChI key: FHURKTIGZAIQGR-BQCLNCLCSA-N
• Gene Information: human ... CARD8(22900), CASP8(841), CASP8AP2(9994), CFLAR(8837), GZMB(3002) ; mouse ... CFLAR(117279), GZMB(171528) ; rat ... CASP8(12370), CFLAR(12633), GZMB(14939), RGD1561819(502004), RGD1562700(502007)

Description

Amino Acid Sequence

NAc-Ile-Glu-Thr-Asp-pNA

Application

Chromogenic substrate for caspase 8 and granzyme B.

Biochem/physiol Actions

IETD is the sequence of amino acid residues 172-175 of procaspase 3, which is cleaved during apoptosis to produce the p12 subunit and a p20 peptide that is further cleaved to form the p17 subunit of mature caspase 3.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)