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AC-0334

Sigma-Aldrich

HNF4 alpha P1 (EP366) Rabbit Monoclonal Primary Antibody

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NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

Ig fraction of antiserum

clone

monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:60

shipped in

wet ice

storage temp.

2-8°C

visualization

nuclear

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This Item
HPA004712AC-0334AHPA005438
antibody form

Ig fraction of antiserum

antibody form

affinity isolated antibody

antibody form

Ig fraction of antiserum

antibody form

affinity isolated antibody

clone

monoclonal

clone

polyclonal

clone

monoclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:60

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:60

technique(s)

immunohistochemistry: 1:1000-1:2500

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Hepatocyte nuclear factor-4α (HNF4α) is a member of the nuclear receptor superfamily is critical for the maintenance of epithelial cell function and normal colon physiology. Isoforms of HNF4α are generated by alternative splicing of the P1 and P2 promoter. Isoforms of HNF4α 1-6 originate from P1 promoter while HNF4α 7-12 are derived from the P2 promoter. The P1 and P2 proteins differ in approximately 16-29 amino acids in the N-terminal domain. HNF4α is critical for the balance between proliferation and differentiation. In normal tissues, HNF4α P1 is localized to the nucleus and expressed in the small intestine, colon, liver, and kidney, but no expression was found in stomach and lung. HNF4α P1 may be used to identify metastatic tumors to the lung. In gastric carcinomas, intestinal, mixed and null types showed HNF4α P1 positive, but the gastric type was HNF4α P1 negative. In contrast, HNF4α P2 was expressed in all gastric carcinomas. HNF4α P1 antibody may be useful for subclassification of gastric tumors. In several studies focusing on colorectal carcinoma, HNF4α P1 was shown to be frequently lost in tumor cells. In contrast, HNF4α P2 expression was unaffected.

Quality


IVD

IVD

IVD

RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and &lt0.1% Sodium Azide

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
IVDorder@ABCAM.com

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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