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AEC Staining Kit






sufficient for ≥1,000 tests (150 ml substrate solution)

storage temp.



AEC Staining Kit has been used as a substrate
  • for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods
  • for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay
  • for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads


Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.


Stable at least one year at 2-8 °C.

Signal Word


Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class Code

3 - Flammable liquids

Flash Point(F)

134.6 °F - closed cup

Flash Point(C)

57 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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Kuangda Lu et al.
Journal of the American Chemical Society, 138(38), 12502-12510 (2016-08-31)
Photodynamic therapy (PDT) can destroy local tumors and minimize normal tissue damage, but is ineffective at eliminating metastases. Checkpoint blockade immunotherapy has enjoyed recent success in the clinic, but only elicits limited rates of systemic antitumor response for most cancers
Leslie R Morse et al.
Translational stroke research, 2(4), 643-650 (2012-03-01)
Spinal cord injury is associated with rapid bone loss and arrested long bone growth due to mechanisms that are poorly understood. In this study, we sought to determine the effects of severe T10 contusion spinal cord injury on the sublesional
Identification of early secretory antigen target-6 epitopes for the immunodiagnosis of active tuberculosis
Vincenti D, et al.
Molecular Medicine, 9(3), 105-105 (2003)
Kaiyuan Ni et al.
Chem, 5(7), 1892-1913 (2019-08-07)
Checkpoint blockade immunotherapy (CBI) elicits durable therapeutic responses by blocking T cell inhibitory pathways of tumors with pre-infiltrated T cells and/or high mutational burden to activate antitumor immunity but is ineffective against poorly immunogenic tumors. Immunogenic radiotherapy, photodynamic therapy (PDT)
Uncoupling of inflammation and insulin resistance by NF-kappaB in transgenic mice through elevated energy expenditure
Tang T, et al.
Test, 285(7), 4637-4644 (2010)


Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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