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B0300

Sigma-Aldrich

Betaine solution

5 M, PCR Reagent

Linear Formula:
(CH3)3N+CH2COO
CAS Number:
Molecular Weight:
117.15
Beilstein:
3537113
MDL number:
PubChem Substance ID:

Quality Level

grade

PCR Reagent

form

liquid

packaging

vial of 1.5 mL

concentration

5 M

application(s)

PCR: suitable

color

colorless

Featured Industry

Agriculture

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

C[N+](C)(C)CC([O-])=O

InChI

1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3

InChI key

KWIUHFFTVRNATP-UHFFFAOYSA-N

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General description

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.

Application

Betaine solution has been used as a component of
  • loop-mediated isothermal amplification (LAMP) reactions
  • polymerase chain reaction for genomic DNA amplification
  • quantitative polymerase chain reaction (qPCR) for telomere length analysis
  • in the PCR amplification of CGG repeats in the genomic DNA.

Packaging

1.5mL per vial

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Thanyaornwanya Charoenwijitkul et al.
Clinical biochemistry, 71, 31-37 (2019-06-15)
The 3.7 kb deletion (-α3.7) is the most common form of α+-thalassemia found in multiple populations which can be classified into three subtypes. In order not to mis-identify it, the molecular information within each population is required. We have addressed this...
P N Hengen
Trends in biochemical sciences, 22(6), 225-226 (1997-06-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and...
Betaine chemistry, roles, and potential use in liver disease
Day CR and Kempson SA
Biochimica et biophysica acta. General subjects, 1860(6), 1098-1106 (2016)
Eniko Sajti et al.
Nature immunology, 21(2), 221-231 (2020-01-22)
The lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey lung tissues. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing their molecular identities and functional potential...
Diet quality and telomere length in older Australian men and women
Milte CM, et al.
European Journal of Nutrition, 57(1), 363-372 (2018)

Protocols

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Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

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Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA

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