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MilliporeSigma

B0300

Sigma-Aldrich

Betaine solution

5 M, PCR Reagent

Synonym(s):

TMG, trimethylglycine

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About This Item

Linear Formula:
(CH3)3N+CH2COO
CAS Number:
Molecular Weight:
117.15
Beilstein/REAXYS Number:
3537113
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.52

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Quality Level

form

liquid

packaging

vial of 1.5 mL

concentration

5 M

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

C[N+](C)(C)CC([O-])=O

InChI

1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3

InChI key

KWIUHFFTVRNATP-UHFFFAOYSA-N

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Show Differences

1 of 4

This Item
619621065695B2629
Betaine BioUltra, ≥99.0% (NT)

61962

Betaine

Betaine United States Pharmacopeia (USP) Reference Standard

1065695

Betaine

Betaine ≥98% (perchloric acid titration)

B2629

Betaine

Quality Level

300

Quality Level

200

Quality Level

-

Quality Level

200

form

liquid

form

-

form

-

form

powder or crystals

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

color

colorless

color

-

color

-

color

-

grade

PCR Reagent

grade

-

grade

pharmaceutical primary standard

grade

-

packaging

vial of 1.5 mL

packaging

-

packaging

-

packaging

-

General description

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups.[1] It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs.[2] Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.[3]

Application

Betaine solution has been used as a component of the reaction mixture for:
  • loop-mediated isothermal amplification (LAMP) reactions[4]
  • polymerase chain reaction for genomic DNA amplification[5]
  • quantitative polymerase chain reaction (qPCR) for telomere length analysis[6]
  • in the PCR amplification of CGG repeats in the genomic DNA[7]
  • reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)[8]
  • PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)[9]
  • reverse transcription during library preparation using Smartseq2[10]

Packaging

1.5mL per vial

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

  1. how to prepare a 5M betaine solution in water?

    1 answer
    1. To prepare 5M solution of Betaine in water, dissolve 29.288 grams of Betaine in 50 ml of nuclease-free distilled or deionized water, and the stock solution should be stored at -20°C. There are no specific requirements for heat or sonication during the preparation process, but the stock solution should be filtered through a 0.22 micron filter before use. Additionally, a stir plate and a stir bar may be used if the powder dissolves slowly.

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