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B0300

Sigma-Aldrich

Betaine solution

5 M, PCR Reagent

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Synonym(s):
TMG, trimethylglycine
Linear Formula:
(CH3)3N+CH2COO
CAS Number:
Molecular Weight:
117.15
Beilstein/REAXYS Number:
3537113
MDL number:
PubChem Substance ID:

grade

PCR Reagent

Quality Level

form

liquid

packaging

vial of 1.5 mL

concentration

5 M

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

C[N+](C)(C)CC([O-])=O

InChI

1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3

InChI key

KWIUHFFTVRNATP-UHFFFAOYSA-N

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This Item
B262961962W422312
Betaine solution 5 M, PCR Reagent

B0300

Betaine solution

Betaine ≥98% (perchloric acid titration)

B2629

Betaine

Betaine BioUltra, ≥99.0% (NT)

61962

Betaine

Betaine ≥98%

W422312

Betaine

form

liquid

form

powder or crystals

form

-

form

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

color

colorless

color

-

color

-

color

-

grade

PCR Reagent

grade

-

grade

-

grade

Halal, Kosher

packaging

vial of 1.5 mL

packaging

-

packaging

-

packaging

-

General description

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.

Application

Betaine solution has been used as a component of the reaction mixture for:
  • loop-mediated isothermal amplification (LAMP) reactions
  • polymerase chain reaction for genomic DNA amplification
  • quantitative polymerase chain reaction (qPCR) for telomere length analysis
  • in the PCR amplification of CGG repeats in the genomic DNA
  • reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)
  • reverse transcription during library preparation using Smartseq2

Packaging

1.5mL per vial

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Betaine chemistry, roles, and potential use in liver disease
Day CR and Kempson SA
Biochimica et biophysica acta. General subjects, 1860(6), 1098-1106 (2016)
Thanyaornwanya Charoenwijitkul et al.
Clinical biochemistry, 71, 31-37 (2019-06-15)
The 3.7 kb deletion (-α3.7) is the most common form of α+-thalassemia found in multiple populations which can be classified into three subtypes. In order not to mis-identify it, the molecular information within each population is required. We have addressed this
P N Hengen
Trends in biochemical sciences, 22(6), 225-226 (1997-06-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and
Eniko Sajti et al.
Nature immunology, 21(2), 221-231 (2020-01-22)
The lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey lung tissues. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing their molecular identities and functional potential
Hela Azaiez et al.
Human mutation, 24(4), 305-311 (2004-09-15)
Genetic testing was completed on 1,294 persons with deafness referred to the Molecular Otolaryngology Research Laboratories to establish a diagnosis of DFNB1. Exon 2 of GJB2 was screened for coding sequence allele variants by denaturing high-performance liquid chromatography (DHPLC) complemented

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