B0686

Sigma-Aldrich

Anti-BTK, N-Terminal antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Agammaglobulinemia tyrosine kinase (ATK), Anti-Bruton’s tyrosine kinase
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 75 kDa

species reactivity

human

concentration

~1 mg/mL

application(s)

microarray: suitable
western blot: 1-2 μg/mL using lysates of Ramos (Human Burkitt lymphoma) cell line

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... BTK(695)

General description

BTK is a tyrosine-protein kinase that modulates signaling, growth, and differentiation in B-lymphocytes. BTK also mediates cytoskeletal rearrangements and associates with Pin1 to regulate the cell-cycle dependent expression of BTK. Additionally, BTK signaling cascades are involved in regulation of calcium fluxes and transcription. Genetic alterations in BTK1 have been linked to X-linked aγglobulinemia . Anti-BTK, N-Terminal antibody is specific for human BTK (75 kDa). In immunoblotting, staining of the BTK band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 11-26 of human BTK, conjugated to KLH via a C-terminal added cysteine residue. This sequence differs from the human Tec tyrosine kinase by 2 amino acids.
The immunogen sequence is conserved in mouse, rat and chicken.

Application

Anti-BTK, N-Terminal antibody is suitable for use in western blot (1-2 μg/mL using lysates of Ramos (Human Burkitt lymphoma) cell line) and microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Abdalla J Mohamed et al.
Immunological reviews, 228(1), 58-73 (2009-03-18)
Bruton's agammaglobulinemia tyrosine kinase (Btk) is a cytoplasmic tyrosine kinase important in B-lymphocyte development, differentiation, and signaling. Btk is a member of the Tec family of kinases. Mutations in the Btk gene lead to X-linked agammaglobulinemia (XLA) in humans and...
B F Nore et al.
European journal of immunology, 30(1), 145-154 (1999-12-22)
Bruton's tyrosine kinase (Btk) is a member of the Tec family of protein tyrosine kinases (PTK) characterized by an N-terminal pleckstrin homology domain (PH) thought to directly interact with phosphoinositides. We report here that wild-type (wt) and also a gain-of-function...
Liang Yu et al.
The Journal of biological chemistry, 281(26), 18201-18207 (2006-04-29)
Bruton tyrosine kinase (Btk) is expressed in B-lymphocytes. Mutations in Btk cause X-linked agammaglobulinemia in humans. However, the mechanism of activation and signaling of this enzyme has not been fully investigated. We have here shown that the peptidylprolyl cis/trans isomerase...

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