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B1267

Sigma-Aldrich

Biotin-maleimide

≥95% (TLC), powder

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Synonym(s):
N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide
Empirical Formula (Hill Notation):
C20H29N5O5S
CAS Number:
Molecular Weight:
451.54
PubChem Substance ID:
NACRES:
NA.46

Quality Level

assay

≥95% (TLC)

form

powder

solubility

acetic acid: 20 mg/mL

storage temp.

2-8°C

SMILES string

O=C(CCCCCN1C(=O)C=CC1=O)NNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23

InChI

1S/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1

InChI key

VAPZWEFPQRDVHR-NJSLBKSFSA-N

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This Item
B7639B5768B7542
vibrant-m

B1267

Biotin-maleimide

vibrant-m

B7639

(+)-Biotin hydrazide

vibrant-m

B7542

Maleimide Activated BSA

form

powder

form

powder

form

powder

form

lyophilized powder

solubility

acetic acid: 20 mg/mL

solubility

DMSO: ≤20 mg/mL

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups.

Application

Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides.
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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