Use with nitrocellulose is not recommended, due to the methanol.
B2025
Brilliant Blue G - Colloidal Concentrate
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$107.00
$107.00
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.85
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This Item | B8522 | B0770 | B8647 |
|---|---|---|---|
| form solution | form liquid | form powder | form methanol solution |
| storage temp. room temp | storage temp. 2-8°C | storage temp. room temp | storage temp. room temp |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
General description
Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE).[1] Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction.[2] It is effective in staining anterior capsule in cornea with relatively less toxic effects.[3]
Application
Preparation Note
The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.
Analysis Note
This product has been tested for suitability on SDS-PAGE.
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Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Roy Auty et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
Antonis Ververis et al.
Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here
Julie Ducreux et al.
Bioconjugate chemistry, 19(10), 2088-2094 (2008-09-24)
PEGylation of antibodies is known to increase their half-life in systemic circulation, but nothing is known regarding whether PEGylation can improve the inhibitory potency of antibodies against target receptors. In this paper, we have examined this question using antibodies directed
A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
Thomas K, et al.
Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
Dyballa N and Metzger S
Journal of Visualized Experiments (2009)
Protocols
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
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Can you use Product B2025, Brilliant Blue G, with nitrocellulose?
1 answer-
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What is the solution stability of Product B2025, Brilliant Blue G?
1 answer-
Once diluted the solution should be stable indefinitely. It should be stored in the refrigerator and shaken well before each use. The solution tends to precipitate on standing and heat accelerates precipitation. Therefore, do not leave the bottle at room temperature overnight.
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How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?
1 answer-
The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.
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How would you recommend that I reconstitute Product B2025, Brilliant Blue G?
1 answer-
One bottle of the product makes 1 liter of working solution. The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the detection limit of Product B2025, Brilliant Blue G?
1 answer-
The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins
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