B2531

Sigma-Aldrich

Monoclonal Anti-BrdU antibody produced in mouse

clone BU-33, ascites fluid, Immunohistology Grade

Synonym(s):
Anti Brdu, Anti Brdu Antibody - Monoclonal Anti-BrdU antibody produced in mouse, Anti-Bromodeoxyuridine, Brdu Antibody
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BU-33, monoclonal

species reactivity

wide range

application(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using sections of intestine from mouse or rat treated in vivo with BrdU

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Monoclonal anti-BrdU (immunohistology grade) (mouse IgG1 isotype) is derived from the BU-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with bromodeoxyuridine conjugated to KLH.

Specificity

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Immunogen

bromodeoxyuridine conjugated to KLH.

Application

Monoclonal Anti-BrdU antibody has been used in immunostaining, BrdU incorporation into DNA, immunohistochemistry and uterine labeling index. It has also been used to investigate cell proliferation.

Biochem/physiol Actions

5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Storage and Stability

For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
B8434 Anti-BrdU antibody, Mouse monoclonal
clone BU-33, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
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