B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

• Synonym(s): 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
• Empirical Formula (Hill Notation): C₁₄H₁₆BrNO₆
• CAS Number: 97753-82-7
• Molecular Weight: 374.18
• Beilstein/REAXYS Number: 1550140
• MDL number: MFCD00063691
• UNSPSC Code: 12352200
• PubChem Substance ID: 24891417
• NACRES: NA.85

Properties

• grade: Molecular Biology
• Quality Level: 200
• sterility: non-sterile
• assay: ≥98% (HPLC)
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: DMF: soluble
• suitability: suitable for substrate for β-galactosidase
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
• InChI key: LINMATFDVHBYOS-MBJXGIAVSA-N

Description

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis,[1] Dictyostelium samples[2] and in the staining of vena cava of adult mice.[3]

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)