B3523
Anti-Human IgG3−Biotin antibody, Mouse monoclonal
clone HP-6050, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-Human IgG3
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
secondary antibodies
clone
HP-6050, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
direct ELISA: 1:40,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Human IgG consists of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physicochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies.
Monoclonal Anti-Human IgG3 (mouse IgG1 isotype) is derived from the hybridoma (HP-60501) produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificity
The antibody is specific for human IgG3 and is non-reactive with other IgG subclasses. This clone has been recommended by the IUIS/WHO study for its specificity and reactivity in a wide range of assay protocols and techniques.
Immunogen
Purified myeloma human IgG3 coupled to polyaminostyrene (PAS) microbeads was used as the immunogen
application
The product may be used for the identification of human IgG3 subclass by means of various immunoassays and ELISA. It has also been used in immunoblotting.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG3 antibody allows for the binding of various labels such as avidin or streptavidin.
Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes. IgG3 has O-glycosylation sites and mediates, phagocytosis, antibody-mediated cellular cytotoxicity, neutralization, and activates complement pathways. IgG3 subtypes have short in vivo half-life and mediates the effector functions than IgG1.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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