B354-05

Sigma-Aldrich

Bovine Aortic Smooth Muscle Cells: BAOSMC (Cryovial)

NACRES:
NA.81

biological source

bovine artery (USDA-inspected cattle)

packaging

pkg of 500,000 cells

growth mode

Adherent

karyotype

2n = 60

morphology

smooth muscle

application(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Bovine large artery smooth muscle cells (BAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.

BAOSMC have been utilized in a number of research studies, for example, to:

  • Elucidate RhoA-dependent serum response signaling pathways (Gudi, 2002); the role of FAK in thrombospondin-1 induced migration of vascular smooth muscle cells (Wang, 2008)
  • Demonstrate the relationship between the disturbed flow and proliferation of smooth muscle cells, leading to intima hyperplasia (Fan, 2010); and study interactions between lipoproteins and extracellular matrix, as well as mechanisms of lipoprotein aggregation contributing to atherosclerosis (Sakr, 2001)
  • Construct an expression cassette to maximize targeted transgene expression (Dronadula, 2010)
  • Test new technologies, such as on-chip contact imaging technique (Liu, 2004; Ji, 2005, 2007; Prakash, 2007), handheld fluorometers (Nelson, 2009) and a “nose on a chip” olfactory sensor (Patel, 2011)
  • Study the behavior of smooth muscle cells in 3d environment (Du, 2008) and smooth muscle/endothelial co-cultures (Niwa, 2007; Fan, 2008; Mathura, 2013); describe an origami-like method of generating three-dimensional (3D) cell-laden microstructures (Kuribayashi-Shigetomi, 2012); and design materials for tissue engineering and cardiovascular implants (Mathews, 2006, 2008)


Characterization: positive for smooth muscle cell specific alpha-actin expression.




Cell Line Origin

Artery

Application

aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation

Components

Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the BAOSMC Culture Protocol.

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Protocols
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.