Anti-Human IgG4−Biotin antibody, Mouse monoclonal

clone HP-6025, purified from hybridoma cell culture

Monoclonal Anti-Human IgG4

Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

secondary antibodies


HP-6025, monoclonal


buffered aqueous solution

species reactivity



direct ELISA: 1:60,000




biotin conjugate

shipped in

dry ice

storage temp.


Looking for similar products? Visit Product Comparison Guide

Related Categories


Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.


The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.
Anti-Human IgG4−Biotin antibody, Mouse monoclonal has been used in immunohistochemistry and immunostaining.
Monoclonal Anti-Human IgG4-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:15,000. It was used for detection of serum IgG4 produced in response to malaria parasite infection by ELISA.

Biochem/physiol Actions

IgG4 is the least abundant immunoglobulin present in the human serum. IgG4 is implicated in a unique process called fab-arm exchange (FAE) and limits the formation of immune complex. It has anti-inflammatory properties. IgG4 functions as a therapeutic antibody. It provides protection against allergy, prevents mast cell degranulation and has a harmful role in malignant melanoma. IgG4 may be associated with autoimmune diseases such as, autoimmune pancreatitis (AIP) type 1 and 2, Mikulicz syndrome, Kuttner′s tumor, Riedel′s thyroiditis and tubulointerstitial nephritis.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG4 antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Nnaemeka C Iriemenam et al.
Vaccine, 27(1), 62-71 (2008-11-04)
Despite many intervention programmes aimed at curtailing the scourge, malaria remains a formidable problem of human health. Immunity to asexual blood-stage of Plasmodium falciparum malaria is thought to be associated with protective antibodies of certain immunoglobulin classes and subclasses. We...
Human IgG4: a structural perspective
Davies AM and Sutton BJ
Immunological Reviews, 268(1), 139-159 (2015)
Amre Nasr et al.
BMC immunology, 14, 38-38 (2013-08-15)
C-reactive protein (CRP) is a nonspecific, acute-phase protein that rises in response to infectious and non-infectious inflammatory processes. Infections are the single largest cause of neonatal deaths globally.The primary aim of this study is to examine the association between CRP...
IgG4-related autoimmune diseases: Polymorphous presentation complicates diagnosis and treatment
Kleger A, et al.
Deutsches Arzteblatt International, 112(8), 128-128 (2015)
Intranasal air sampling in homes: relationships among reservoir allergen concentrations and asthma severity
Gore RB, et al.
The Journal of Allergy and Clinical Immunology, 117(3), 649-655 (2006)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.