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B4639

Sigma-Aldrich

Biotin

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

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Synonym(s):
D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
Empirical Formula (Hill Notation):
C10H16N2O3S
CAS Number:
Molecular Weight:
244.31
Beilstein/REAXYS Number:
86838
EC Number:
MDL number:
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.21

product line

BioReagent

Quality Level

assay

≥99%

form

powder

mol wt

Mw 244.31 g/mol

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

color

white to off-white

mp

231-233 °C (lit.)

solubility

H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)

storage temp.

2-8°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

InChI key

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

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1 of 4

This Item
14400B0301B4501
vibrant-m

B4639

Biotin

Essential+ Grade
vibrant-m

14400

Biotin

-
vibrant-m

B0301

Biotin

-
vibrant-m

B4501

Biotin

-
assay

≥99%

assay

≥99.0% (T)

assay

97.5-102.0%

assay

≥99% (HPLC)

color

white to off-white

color

colorless to white

color

white

color

white to off-white

solubility

H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)

solubility

alcohol: soluble 80mg/100 ml at 25 °C, water: soluble 22mg/100 ml at 25 °C, 0.1 M NaOH: 10 mg/mL at 25 °C, clear, colorless, H2O: very soluble, organic solvents: insoluble

solubility

-

solubility

ammonium hydroxide: 50 mg/mL (2 M)

mp

231-233 °C (lit.)

mp

229-234 °C, 231-233 °C (lit.)

mp

231-233 °C (lit.)

mp

231-233 °C (lit.)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

General description

Biotin is also called as B complex vitamin. It is a bicyclic compound, which has a molecular weight of 244.31 Da. Biotin serves as an important cofactor for mammalian carboxylases. It is found within the cells in free and bound pools.

Application

Biotin has been used:
  • as a M199 media supplement in in vitro culture system
  • as a component of SATO serum free medium for the isolation of cortical oligodendrocyte precursors (OPCs)
  • in biochemical precursors culture for de novo fatty acid synthesis
  • as a component in aggregate culture media

Biochem/physiol Actions

Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Preparation of rat brain aggregate cultures for neuron and glia development studies
Koito H and Li J
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Convergent functional genomics of oligodendrocyte differentiation identifies multiple autoinhibitory signaling circuits
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Articles

Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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