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B4679

Sigma-Aldrich

BIS-TRIS propane

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):
1,3-Bis[tris(hydroxymethyl)methylamino]propane
Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
Beilstein:
1786109
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

Assay

≥99.0%

form

crystals

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and bioburden, tested

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

application(s)

diagnostic assay manufacturing

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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This Item
B6755RDD014B4429
BIS-TRIS propane BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

B4679

BIS-TRIS propane

BIS-TRIS propane ≥99.0% (titration)

Sigma-Aldrich

B6755

BIS-TRIS propane

BIS-TRIS propane anhydrous, free-flowing, Redi-Dri™, ≥99.0%

Sigma-Aldrich

RDD014

BIS-TRIS propane

BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

Sigma-Aldrich

B4429

BIS-TRIS

assay

≥99.0%

assay

≥99.0% (titration)

assay

≥99.0%

assay

≥98.0%

form

crystals

form

powder

form

powder

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

impurities

endotoxin and bioburden, tested

impurities

-

impurities

≤0.2% water (Karl Fischer), ≤0.5% tris(hydroxymethyl aminomethane (H-NMR)

impurities

endotoxin and total aerobic microbial count, tested

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

mp

-

Other Notes

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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S J Smith et al.
The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)
The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)
Michael C Breadmore
Journal of chromatography. A, 1217(24), 3900-3906 (2010-05-11)
An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could
R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
R Coronado et al.
The Journal of general physiology, 79(4), 529-547 (1982-04-01)
A collection of organic cations has been used to probe the gross structural features of the ionic diffusion pathway in a K+-selective channel from sarcoplasmic reticulum (SR). Channels were incorporated into planar phospholipid bilayer membranes, and single-channel currents were measured
M H Hopkins et al.
Journal of enzyme inhibition, 6(3), 195-199 (1992-01-01)
It is hypothesized that buffers capable of forming a Schiff base with the PLP of gamma-aminobutyric acid aminotransferase (GABA-AT) may lead to denaturation and inactivation of the enzyme. On the basis of this hypothesis three new methods for the selective

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