The properties and content of this product have not been determined. The percentage of protein is lot specific. The Certificate of Analysis (CoA) provides information on the quality control release tests.
B4882
Bromelain from pineapple stem
≥3 units/mg protein
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10 G
$98.90
25 G
$185.00
100 G
$602.00
500 G
$2,010.00
1 KG
$3,580.00
$98.90
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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form
lyophilized powder
Quality Level
specific activity
≥3 units/mg protein
composition
Protein, ≥30% biuret
storage temp.
−20°C
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Related Categories
1 of 4
This Item | B5144 | C9584 | E0885 |
|---|---|---|---|
| specific activity ≥3 units/mg protein | specific activity (chromatography purified), ≥4 units/mg protein | specific activity ≥125 units/mg protein | specific activity ≥100 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, ≥30% biuret | composition Protein, 30-50% biuret | composition Protein, 40-70% | composition Protein, ≥20% Lowry |
Application
Bromelain from pineapple stem has been used:
- to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants[1]
- as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay[2]
- to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences[3]
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen [4].
Biochem/physiol Actions
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Preparation Note
The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.
Other Notes
One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Soghra Khatun Haq et al.
European journal of biochemistry, 269(1), 47-52 (2002-01-11)
Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, fluorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stem bromelain lacks a well defined
Enkephalin related peptides are released from jejunum wall by orally ingested bromelain
Orlandi-Mattos PE, et al.
Peptides, 115, 32-42 (2019)
In vitro antiproliferative activity of fresh pineapple juices on ovarian and colon cancer cell lines
Gani MBA, et al.
International Journal of Peptide Research and Therapeutics, 21(3), 353-364 (2015)
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente FA, et al.
Process. Biochem., 51(4), 528-534 (2016)
A D Rowan et al.
The Biochemical journal, 266(3), 869-875 (1990-03-15)
The pineapple plant (Ananas comosus) was shown to contain at least four distinct cysteine proteinases, which were purified by a procedure involving active-site-directed affinity chromatography. The major proteinase present in extracts of plant stem was stem bromelain, whilst fruit bromelain
Protocols
Protocol for HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column
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I WILL USE BROMELAIN (B4882-10G ).IN A RESEARCH PROJECT. BROMELAIN IS COMPOSED OF MANY ENZYMES. THEY WERE ASKED WHAT the PROPERTIES AND CONTENT OF THE BROMELAIN YOU USE. I WOULD BE HAPPY IF YOU HELPED ME WITH
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