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B5002

Sigma-Aldrich

5-Bromo-2′-deoxyuridine

≥99% (HPLC)

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Synonym(s):
5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
Empirical Formula (Hill Notation):
C9H11BrN2O5
CAS Number:
Molecular Weight:
307.10
Beilstein:
30395
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.51

biological source

synthetic (organic)

Assay

≥99% (HPLC)

form

powder

mp

191-194 °C (dec.) (lit.)

solubility

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

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This Item
B9285D5412I7125
2′-Deoxyuridine ≥98.5%

Sigma-Aldrich

D5412

2′-Deoxyuridine

assay

≥99% (HPLC)

assay

≥99%

assay

≥98.5%

assay

≥99% (HPLC)

form

powder

form

powder

form

powder

form

powder or crystals

mp

191-194 °C (dec.) (lit.)

mp

191-194 °C (dec.) (lit.)

mp

-

mp

194 °C (lit.)

solubility

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

solubility

NH4OH: 0.1 M at 20 °C, clear, colorless

solubility

water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

solubility

1 N NH4OH: 50 mg/mL, clear to very slightly hazy, colorless to light yellow

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

Application

5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
5-Bromo-2′-deoxyuridine has been used:
  • as a supplement to study the effect of electroconvulsive seizures on hippocampal neurogenesis
  • as a supplement to study the loss-of-gene function in adult zebrafish heart
  • in T lymphocyte proliferation assay{133]

Biochem/physiol Actions

5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation, because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.
Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Muta. 1B - Repr. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Mechanisms of Differentiation, Volume 2, 85-85 (1990)
Target Validation in Drug Discovery, 109-109 (2011)
Electroconvulsive seizures increase hippocampal neurogenesis after chronic corticosterone treatment
Hellsten J, et al.
The European Journal of Neuroscience, 16(2), 283-290 (2002)
PEG-PLA nanoparticles facilitate siRNA knockdown in adult zebrafish heart
Diao J, et al.
Developmental Biology, 406, 196-202 (2015)
Francisco Alén et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Mutant mice with respect to the splicing factor Zrsr1 present altered spermatogenesis and infertility. To investigate whether Zrsr1 is involved in the homeostatic control that the hypothalamus exerts over reproductive functions, we first analyzed both differential gene and isoform expression

Articles

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage process in which the cell 1) increases in size (G1-stage), 2) copies its DNA (synthesis, S-stage), 3) prepares to divide (G2-stage), and 4) divides (mitosis, M-stage). Due to their anionic nature, nucleoside triphosphates (NTPs), the building blocks of both RNA and DNA, do not permeate cell membranes.

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