MilliporeSigma
All Photos(3)

B5283

Sigma-Aldrich

Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

Synonym(s):
Monoclonal Anti-Rabbit IgG (γ-chain specific)
NACRES:
NA.46

biological source

mouse

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-96, monoclonal

form

buffered aqueous solution

species reactivity

rabbit

should not react with

chicken, guinea pig, goat, horse, sheep, rat, bovine, human, pig, feline, canine

technique(s)

direct ELISA: 1:150,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
western blot (chemiluminescent): 1:200,000-1:400,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother′s milk. The variable region of IgG antibody is specific to antigens and is highly conserved.

Application

Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Regulatory function of whey acidic protein in the proliferation of mouse mammary epithelial cells in vivo and in vitro.
Nukumi N, et al.
Developmental Biology, 274(1), 31-44 (2004)
Yoann Aldon et al.
Cell reports, 24(12), 3324-3338 (2018-09-21)
The HIV-1-envelope glycoprotein (Env) is the main target of antigen design for antibody-based prophylactic vaccines. The generation of broadly neutralizing antibodies (bNAb) likely requires the appropriate presentation of stabilized trimers preventing exposure of non-neutralizing antibody (nNAb) epitopes. We designed a
Retained features of embryonic metabolism in the adult MRL mouse.
Naviaux RK, et al.
Molecular Genetics and Metabolism, 96(3), 133-144 (2009)
Placental transfer of IgG subclasses in a Japanese population.
Hashira S, et al.
Pediatrics International : Official Journal of the Japan Pediatric Society, 42(4), 337-342 (2000)
β-Catenin Signaling Promotes Proliferation of Progenitor Cells in the Adult Mouse Subventricular Zone.
Kazuhide A, et al.
Stem Cells, 25(11), 2827-2836 (2007)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service