B5285

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt

≥98%

• Synonym(s): BC-Indicator, X-GlcA, X-glucuronide
• Empirical Formula (Hill Notation): C₁₄H₁₂BrClNNaO₇
• CAS Number: 129541-41-9
• Molecular Weight: 444.59
• MDL number: MFCD00135782
• PubChem Substance ID: 24891832

Properties

• Quality Level: 200
• Assay: ≥98%
• form: powder
• solubility: water: 50 mg/mL, clear, colorless
• storage temp.: −20°C
• SMILES string: [Na+].O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C([O-])=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1
• InChI key: IBLSVGDGSKUDCT-CYRSAHDMSA-M

Description

Application

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt has been used:

• as a component in β-glucuronidase (GUS) assay buffer for histochemical GUS assay
• to prepare X-Gluc or GUS staining solution
• to confirm GUS expression in samples by X-gluc staining

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colorless substrate for β-glucuronidase enzyme, generates a blue color at the end of the reaction.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves