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B5388

Sigma-Aldrich

Brilliant Cresyl Blue ALD

certified by the Biological Stain Commission

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Synonym(s):
3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C, Brilliant Cresyl blue
Linear Formula:
C17H20N3OCl · 1/2ZnCl2
CAS Number:
Molecular Weight:
385.96
Colour Index Number:
51010
Beilstein:
5690713
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥60%

solubility

water: 1 mg/mL, blue to very deep blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

SIHASHFMDFXGSX-UHFFFAOYSA-J

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This Item
860867B7920B0149
Brilliant Blue R pure

Sigma-Aldrich

B7920

Brilliant Blue R

Brilliant Blue R Dye content ~50 %, Technical grade

Sigma-Aldrich

B0149

Brilliant Blue R

form

powder

form

powder or crystals

form

powder

form

powder

composition

Dye content, ≥60%

composition

Dye content, ≥50%

composition

-

composition

Dye content, ~50%

solubility

water: 1 mg/mL, blue to very deep blue

solubility

-

solubility

H2O: 1 mg/mL

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

Biochem/physiol Actions

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Suitability

Certified as a supravital stain for reticulocytes.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Vitrification of immature and matured bovine oocytes: effect of brilliant cresyl blue selection and hyaluronan addition.
Animal Reproduction, 13 (2016)
Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation.
Rodriguez-Gonzalez E, et al.
Reproduction, Nutrition, Development, 43, 179-179 (2003)
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Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can
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