B5388

Sigma-Aldrich

Brilliant Cresyl Blue ALD

certified by the Biological Stain Commission

Synonym(s):
Brilliant Blue C, 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Cresyl blue
Linear Formula:
C17H20N3OCl · 1/2ZnCl2
CAS Number:
Molecular Weight:
385.96
Colour Index Number:
51010
Beilstein/REAXYS Number:
5690713
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥60%

application(s)

hematology: suitable
histology: suitable

solubility

water: 1 mg/mL, blue to very deep blue

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

Inchi Code

1S/2C17H20N3O.CH3.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;;/h2*6-10H,4-5,18H2,1-3H3;1H3;4*1H;/q2*+1;;;;;;+2/p-4

InChI key

BSALPDUPRKIDTE-UHFFFAOYSA-J
SIHASHFMDFXGSX-UHFFFAOYSA-J

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

Application

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

Packaging

10 g in glass bottle

Biochem/physiol Actions

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Suitability

Certified as a supravital stain for reticulocytes.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation.
Rodriguez-Gonzalez E, et al.
Reproduction, Nutrition, Development, 43, 179-179 (2003)
Vitrification of immature and matured bovine oocytes: effect of brilliant cresyl blue selection and hyaluronan addition.
Animal Reproduction, 13 (2016)
Liqin Wang et al.
Journal of biomedicine & biotechnology, 2012, 161372-161372 (2012-06-08)
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can...
G M Bragança et al.
Domestic animal endocrinology, 74, 106518-106518 (2020-07-28)
This study evaluated the role of progesterone (P4) and medroxyprogesterone acetate (MAP) on the molecular status of immature cumulus-oocyte complexes (COCs) and the implications for oocyte quality in sheep. The number of viable COCs per ewe and the rate of...
David Berthier et al.
PloS one, 10(5), e0126498-e0126498 (2015-05-09)
Animal African Trypanosomosis particularly affects cattle and dramatically impairs livestock development in sub-Saharan Africa. African Zebu (AFZ) or European taurine breeds usually die of the disease in the absence of treatment, whereas West African taurine breeds (AFT), considered trypanotolerant, are...

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