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B5525

Sigma-Aldrich

Bromophenol Blue sodium salt

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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$45.10
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$64.00
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$162.00

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About This Item

Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
34725-61-6
Molecular Weight:
691.94
Beilstein/REAXYS Number:
6995701
EC Number:
252-170-2
MDL number:
MFCD00013793
UNSPSC Code:
12171500
PubChem Substance ID:
57653941
NACRES:
NA.52

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form

powder

Quality Level

200

technique(s)

electrophoresis: suitable

solubility

water: soluble

suitability

suitable for nucleic acid electrophoresis

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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This Item
B8026318744114405
Bromophenol Blue sodium salt

Sigma-Aldrich

B5525

Bromophenol Blue sodium salt

Bromophenol Blue sodium salt Molecular Biology, suitable for electrophoresis

Sigma-Aldrich

B8026

Bromophenol Blue sodium salt

Bromophenol Blue solution 0.04 wt. % in H2O

Sigma-Aldrich

318744

Bromophenol Blue solution

Bromophenol Blue sodium salt Dye content 90 %, ACS reagent

Sigma-Aldrich

114405

Bromophenol Blue sodium salt

suitability

suitable for nucleic acid electrophoresis

suitability

suitable for (Suitable For Use as a Tracking Dye For Electrophoresis in Alkaline and Neutral Buffers)

suitability

-

suitability

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

titration: suitable

technique(s)

titration: suitable

form

powder

form

powder

form

liquid

form

powder

solubility

water: soluble

solubility

-

solubility

-

solubility

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.
Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000483860
0000483860
0000483663
0000483663
0000382212

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