The word borate is probably being used to describe a borate buffer. Borate buffers are typically used around pH 9. One could prepare a sodium borate buffer by dissolving this boric acid product in water (to give a solution at approximately pH 5) followed by the addition of sodium hydroxide to bring it up to pH 9. But the more typical approach is to use Sodium tetraborate decahydrate (e.g., Product B9876). Dissolving this Sodium tetraborate decahydrate in water will give a solution that has a pH of approximately 9.3. Then, the Sodium dodecyl sulfate (SDS) could be added to that solution.
B6768
Boric acid
BioReagent, Molecular Biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
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About This Item
Linear Formula:
H3BO3
CAS Number:
Molecular Weight:
61.83
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
38120104
PubChem Substance ID:
NACRES:
NA.25
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grade
Molecular Biology
Quality Level
vapor pressure
2.6 mmHg ( 20 °C)
product line
BioReagent
assay
≥99.5%
form
powder
concentration
≥99.5%
technique(s)
cell culture | mammalian: suitable
cell culture | plant: suitable
pH
5.1 (25 °C, 1.8 g/L)
mp
160 °C (dec.) (lit.)
solubility
water: 40 mg/mL, clear, colorless
density
1.49 g/cm3 at 23 °C
cation traces
Fe: ≤5 ppm
Mg: ≤5 ppm
heavy metals (as Pb): ≤10 ppm
UV absorption
λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.05
foreign activity
DNase, RNase, and protease, none detected
storage temp.
room temp
SMILES string
OB(O)O
InChI
1S/BH3O3/c2-1(3)4/h2-4H
InChI key
KGBXLFKZBHKPEV-UHFFFAOYSA-N
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1 of 4
This Item | B0394 | B7901 | B0252 |
|---|---|---|---|
| grade for molecular biology | grade ACS reagent | grade - | grade - |
| assay ≥99.5% | assay ≥99.5% | assay ≥99.5% | assay ≥99.5% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| pH 5.1 (25 °C, 1.8 g/L) | pH - | pH 8.2-10.1 | pH - |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. - |
General description
Boric acid is a weak monobasic Lewis acid (electron pair acceptor), which reacts reversibly with alcohols. It is used in dermal and women′s hygiene products due to its mild antibacterial and antifungal effects. Boric acid is used as a buffer in ophthalmic products. It acts as an effective solid lubrication agent because of its molecular structure. Boric acid finds its applications in glass making, fire retardant. Boric acid is used in biochemistry in buffer solutions. It also forms complex equilibria in solution, with the tetraborate ion (B4O7 2- ) being the predominant species. Borate buffers are involved in discontinuous electrophoresis and capillary electrophoresis systems for the separation of proteins and nucleic acids.
Application
Boric acid has been used:
- to prepare boric buffer for the quantification of primary amine groups at the surface of nanocapsules
- to study its effects on epigenetic change
- for the estimation of protein in wheatgrass using Kjeldahl method
Used to promote amidations of carboxylic acids and amines in catalytic amounts. This technology avoids the requirement of preactivation of the carboxylic acid or use of coupling reagents.
Intensified Azeotropic Distillation: A Strategy for Optimizing Direct Amidation
Intensified Azeotropic Distillation: A Strategy for Optimizing Direct Amidation
Features and Benefits
- Environment-friendly
- Non-toxic
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Mehmet Ozansoy et al.
Turkish journal of biology = Turk biyoloji dergisi, 44(4), 208-214 (2020-09-15)
Boron is a naturally occurring trace element found in organic and inorganic complexes. Boron-containing compounds are required for living organisms for diverse metabolic functions, including nitrogen fixation in microorganisms, cell wall stability in plants, and bone and carbohydrate metabolism in
Calvin S Carter et al.
Cell metabolism, 32(4), 561-574 (2020-10-08)
Aberrant redox signaling underlies the pathophysiology of many chronic metabolic diseases, including type 2 diabetes (T2D). Methodologies aimed at rebalancing systemic redox homeostasis have had limited success. A noninvasive, sustained approach would enable the long-term control of redox signaling for
Joy E Kirkpatrick et al.
Epigenetics, 13(5), 557-572 (2018-06-22)
Periodontal disease (PD) afflicts 46% of Americans with no effective adjunctive therapies available. While most pharmacotherapy for PD targets bacteria, the host immune response is responsible for driving tissue damage and bone loss in severe disease. Herein, we establish that
C A Yoder et al.
Poultry science, 85(7), 1285-1293 (2006-07-13)
Nicarbazin (NCZ) is an anticoccidial drug routinely used in the poultry industry that can negatively affect reproduction by reducing egg production, egg weight, and egg hatchability. The molecular mechanisms by which NCZ affects reproduction are unknown. Lipoprotein lipase, vitellogenin, transglutaminase
Jing Li et al.
American journal of physiology. Cell physiology, 306(10), C943-C960 (2014-03-22)
The human solute carrier (SLC26) family of anion transporters consists of 10 members (SLCA1-11, SLCA10 being a pseudogene) that encode membrane proteins containing ~12 transmembrane (TM) segments with putative N-glycosylation sites (-NXS/T-) in extracellular loops and a COOH-terminal cytosolic STAS
Related Content
Product Information Sheet
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Hello, I am working on a tissue clearance project with this product. From the literature review, I noticed the use of 4% (wt/vol) SDS to 400mM borate as a crucial step. In the literature, there are ambiguous references to either borate or boric acid.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I find out the expiration date? Lot No : BCCH1940
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Expiration information will be on the certificate of analysis. The certificate of analysis can be accessed by clicking on the "COO/COA" tab under the product photo the product detail page or the scrolling down to the documentation section. Here is a copy of lot BCCH1940 COA: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/B6768-BULKBCCH1940.pdf.
This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services
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