Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
8B - Non-combustible, corrosive hazardous materials
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples.
The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.
he protein-dye complex is stable up to 60 minutes. The absorbency of the samples must be recorded before the 60 minute time limit and within 10 minutes of each other.
The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 μg of total protein in 1 mL).
No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
The emphasis of this page is on the rules and good practice in sample preparation for Western Blots.
A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.