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B6917

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, suitable for (for molecular biology), Non-acetylated

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

grade

for molecular biology

Assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

quality

deacetylated

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

glass bottle of

origin

USA origin

technique(s)

electrophoresis: suitable-98% using Agarose

impurities

enzymatic impurity, none detected

pH

7

solubility

water: soluble (50 mg/ml)

suitability

suitable for (for molecular biology)

UniProt accession no.

foreign activity

Alkaline Phosphatase free
Alkaline phosphatase and peroxidase, none detected
DNase free
DNase, RNase, and protease, none detected
Peroxidase free
Protease free
RNase free

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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This Item
B4287A7638A7511
Bovine Serum Albumin lyophilized powder, suitable for (for molecular biology), Non-acetylated

Sigma-Aldrich

B6917

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

Sigma-Aldrich

B4287

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

Sigma-Aldrich

A7638

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially fatty acid free, ≥97% (agarose gel electrophoresis), 1X Crystallized

Sigma-Aldrich

A7511

Bovine Serum Albumin

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

assay

≥98% (agarose gel electrophoresis)

assay

≥98%

assay

≥99% (agarose gel electrophoresis)

assay

≥97% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

cold ethanol and heatshock fractionation

purified by

cold ethanol fractionation

General description

Bovine Serum Albumin (BSA) is a plasma protein produced in the bovine liver that has a globular, non-glycosylated α-helical structure. It consists of a single polypeptide chain with 583 amino acid residues and 17 cysteine residues that form the cross-linked disulfide bridges, stabilizing its structure. The BSA protein comprises three structurally distinct domains (I, II, and III), each of which contains two sub-domains (A and B) arranged in a heart-shaped architecture.

Application

Bovine Serum Albumin (BSA) has been used
  • as a component of phosphate buffer saline (PBS) solution for dissolving tumor necrosis factor α (TNFα), which was added as a supplement for culture media
  • as a blocking agent to reduce non-specific binding during the albumin sensor assay
  • as a blocking agent in the immunofluorescence (IF) assay
  • as a protein standard in the Bradford assay

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a versatile protein commonly utilized in cell and tissue culture media as a supplement. It helps in lipid solubilization and stabilizing other proteins. BSA is also a carrier for small molecules such as steroids, fatty acids, and thyroid hormones. It serves as a standard protein for immunochemical tests to determine protein concentration in a solution and calibration studies.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

This BSA product for molecular biology is tested for the following properties:
* Alkaline Phosphatase-free
* DNase-free / Exonuclease-free
* Nickase-free / Endonuclease-free
* Peroxidase-free
* Protease-free
* RNase-free

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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