All Photos(1)

B7435

Sigma-Aldrich

CelLytic B Cell Lysis Reagent

For bacterial cell lysis, standard strength

MDL number:

Quality Level

Looking for similar products? Visit Product Comparison Guide

Application

CelLytic B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.

Features and Benefits

  • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
  • Scalable for 1 to 25 grams of bacterial cell paste
  • No interference with downstream applications such as affinity chromatography, IP, and Western blotting
  • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Other Notes

Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What bacterial cells can be used with CelLytic B?

    CelLytic B Reagent by itself (no lysozyme or benzonase) was optimized for E. coli strain BL21.  It also works with other common E. coli strains such as DH5?, JM109, and HB 101. The CelLytic B Reagent may be used on other similar bacterial cells. The CelLytic B Plus Kit is compatible with many more difficult to lyse Gram positive bacteria such as Bacillus and Streptococcus.

  6. What is the difference between CelLytic B (Product Nos. B7435, B7310, C8740) and CelLytic B Plus  (Product Nos. CB0050 and CB0500)?

    CelLytic B Plus contains CelLytic B, Product No. B7435 as well as lysozyme, benzonase, and a protease inhibitor cocktail for a complete reagent for cell lysates for protein isolation.

  7. What is the difference between CelLytic B (Product No. B7435), CelLytic B 2x (Product No. B7310) and CelLytic B 10x (Product No. C8740)?

    CelLytic B 2X has double the strength of CelLytic B for small volume extractions. This allows CelLytic B 2X to be used when a higher protein concentration or lower volume is required. The original formula, CelLytic B, requires 10-20 ml of the reagent for 1 gram of wet cell paste.  Only 5 ml of the CelLytic B 2X reagent is required to lyse and extract protein from 1 gram of wet cell paste.  CelLytic B 10X is provided as a concentrated mixture of the proprietary detergents found in CelLytic B, without any buffering component. This allows the user to customize the extraction reagent by choosing the detergent concentration and buffering components ideal for the extraction and purification of the protein(s) of interest. CelLytic B 10X is also suitable for the lysis of yeast and mammalian cells.  

  8. Can I obtain protein from inclusion bodies with CelLytic B?

    If the protein is found in inclusion bodies, you need to use an additional reagent, Product No. C5236, CelLytic IB to solubilize the proteins from the inclusion bodies.  CelLytic B is used to isolate the inclusion bodies from the bacteria.  CelLytic IB, Product No. C5236 can then be used to solubilize the proteins from the inclusion bodies.

  9. Can I use the CelLytic B cell lysate in an activity assay?

    CelLytic B contains a zwitterionic detergent so the protein is active and can be used in protein activity assays.

  10. Can I concentrate my sample that was made using CelLytic B?

    If concentrating a sample, be aware that concentration using filter concentrators may increase the detergent concentration significantly and cause issues in downstream applications.  heoretically, ways to get around this are TCA precipitation, or diluting the extract below the CMC prior to concentration. This would be at least a 1/5 dilution, based on the following reference: Saito, S., and Tsuchiya, T., Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry. Biochem. J., 222(3), 829-832, 1984.

  11. Can I use CelLytic B for eukaryotic cells?

    CelLytic B was optimized for bacterial cells.  It may lyse some mammalian cells. CelLytic B 10X is a better choice for the lysis of yeast and mammalian cells due to the increased detergent concentration.

  12. Can I use CelLytic B with the maltose binding protein system (MBP System)?

    CelLytic B cannot be used with the MBP system.  We have used this in the maltose binding protein system, and know that it will NOT work in this system, since any and all detergents will destroy binding of protein to the resin.

  13. When using the CelLytic B lysis reagent, do I need to make a dilution prior to measuring protein by Bradford assay?  

    When using the CelLytic B lysis reagent (B7435, B7310, C8740, and contained in kits CB0050 and CB0500), it is best to use a 1:10 dilution in order to reduce the risk of interference with the Bradford Reagent.  A 1:20 dilution would be even better, as long as the protein level remains within the standard curve. 

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ken Motohashi
BMC biotechnology, 15, 47-47 (2015-06-04)
Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses the components of the λ prophage Red/ET recombination system. This method facilitates restriction endonuclease...
Monika Raab et al.
Scientific reports, 9(1), 10462-10462 (2019-07-20)
While the immune cell adaptor protein SKAP1 mediates LFA-1 activation induced by antigen-receptor (TCR/CD3) ligation on T-cells, it is unclear whether the adaptor interacts with other mediators of T-cell function. In this context, the serine/threonine kinase, polo-like kinase (PLK1) regulates...
Prion-like seeding of misfolded alpha-synuclein in the brains of dementia with Lewy body patients in RT-QUIC
Sano K, et al.
Molecular Neurobiology, 55(5), 3916-3930 (2018)
Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens
Ha SJ, et al.
Bioprocess and Biosystems Engineering, 32(5), 697-700 (2009)
Minho Noh et al.
Cell systems, 5(4), 418-426 (2017-10-02)
Escherichia coli gene expression knockdown using synthetic small RNA (sRNA) can be fine-tuned by altering sRNA sequences to modulate target mRNA-binding ability, but this requires thorough checking for off-target effects. Here, we present an sRNA gene expression knockdown system fine-tuned...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service