B7653
Monoclonal Anti-Biotin antibody produced in mouse
clone BN-34, ascites fluid
Synonym(s):
Monoclonal Anti-Biotin
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
BN-34, monoclonal
Application:
ELISA (c), ELISA (i), IHC (p)
Citations:
41
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BN-34, monoclonal
contains
15 mM sodium azide
technique(s)
capture ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: 1:4,000
isotype
IgG1
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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1 of 4
This Item | A1559 | F4024 | V9505 |
|---|---|---|---|
| antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form ascites fluid |
| clone BN-34, monoclonal | clone BN-34, monoclonal | clone BN-34, monoclonal | clone CD-29, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate agarose conjugate | conjugate FITC conjugate | conjugate unconjugated |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| isotype IgG1 | isotype IgG1 | isotype IgG1 | isotype IgG1 |
General description
Biotin is a water-soluble vitamin. It is also called as vitamin B8. Biotin is a bicyclic compound that has a uredo ring and a tetrahydrothiophene ring.
Monoclonal Anti-Biotin recognizes the free biotin molecule and biotin conjugated to various immunoglobulins in ELISA and immunohistochemical techniques. Specificity was verified by using biotinylated goat antibodies reactive against human and rabbit antigens coated on microwell plates.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Immunogen
Biotin-KLH conjugate
Biotinylated keyhole limpet hemocyanin (KLH)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
May be substituted for avidin to decrease non-specific binding.
Monoclonal Anti-Biotin antibody has been used in proximity ligation assay (PLA), cell surface biotinylation assay and western blotting.
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Utilized in a variety of applications including blotting, immunocytochemistyr, insitu nucleic acid hybridization, ELISA, fluorescent activated cell-sorting (FACS) and electron microscopy.Titer is approximately 1:2000 by ELISA but should be optimized by the individual user for their application of choiceAn essential vitamin, biotin is required by living organisms and cells in culture. Avidin′s high affinity for biotin is often exploited in immuno-based assays. While standard assay methods using the avidin-biotin enzyme complex will suffice for most studies, there are occasions when enhanced sensitivity is needed to detect smaller amounts of antigen or localize low density antigens in histologic sections. Conventional immunoassay methods are improved by the use of Monoclonal Anti-Biotin which enhances the sensitivity of avidin-biotin immunoassays by bridging a second layer of avidin-biotin-enzyme complex. This antibody can be used in many other applications where biotin can be introduced as a target label.
Monoclonal Anti-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:4000 and for western blotting.
Monoclonal Anti-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:4000 and for western blotting.
Biochem/physiol Actions
Biotin may participate in the replication and transcription of DNA. It acts as a coenzyme in the fatty acid biosynthesis, glyconeogenesis, and catabolism of several branched-chain amino acids and odd-chain fatty acids.
Preparation Note
Developed in BALB/c mice immunized with biotinylated KLH. Mouse myeloma cells and splenocytes are fused together to form a BN-34 hybridoma.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Erik C Yusko et al.
Nature nanotechnology, 6(4), 253-260 (2011-02-22)
Synthetic nanopores have been used to study individual biomolecules in high throughput, but their performance as sensors does not match that of biological ion channels. Challenges include control of nanopore diameters and surface chemistry, modification of the translocation times of
Israr-ul H Ansari et al.
The Journal of biological chemistry, 290(38), 23110-23123 (2015-08-05)
The negative charge of phosphatidylserine in lipid bilayers of secretory vesicles and plasma membranes couples the domains of positively charged amino acids of secretory vesicle SNARE proteins with similar domains of plasma membrane SNARE proteins enhancing fusion of the two
Direct visualization of newly synthesized target proteins in situ.
Susanne TD, et al.
Nature Methods, 12(5), 411-411 (2015)
Characterization of P4 ATPase phospholipid translocases (flippases) in human and rat pancreatic beta cells: their gene silencing inhibits insulin secretion.
Israr-ul HA, et al.
The Journal of Biological Chemistry, M115-M115 (2015)
Susanne tom Dieck et al.
Nature methods, 12(5), 411-414 (2015-03-17)
Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique
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