B8400

Monoclonal Anti-Bovine IgG2 antibody produced in mouse

clone BG2-7, ascites fluid

• MDL number: MFCD00164545
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: BG2-7, monoclonal
• contains: 15 mM sodium azide
• technique(s): capture ELISA: suitable; dot blot: suitable; indirect ELISA: 1:1,000; western blot: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

The IgG class antibodies contain two subclasses, IgG1 and IgG2, which have antigenic differences in the Fc portion of the heavy chains. Monoclonal Anti-Bovine IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse.

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG2 antibody has been used in:

• western blot
• enzyme linked immunosorbent assay (ELISA)
• dot blot assay

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable