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Bleomycin sulfate from Streptomyces verticillus

1.5-2.0 units/mg solid, BioReagent, suitable for cell culture

Bleo, Blenoxane, Blexane
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

product line




specific activity

1.5-2.0 units/mg solid


cell culture | mammalian: suitable




H2O: 20 mg/mL, clear, colorless

cation traces

Cu: ≤0.10%

antibiotic activity spectrum


Mode of action

DNA synthesis | interferes

storage temp.


SMILES string




InChI key


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General description

Bleomycin Sulfate is an antineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 μg/mL.
Chemical structure: glycopeptide


Bleomycin is used in combination with Etoposide and Cisplatin to treat testicular, ovarian and cervical cancer. This combination is popular as BEP chemotherapy and has also been demonstrated as effective and most successful chemotherapy regimen in germ-cell tumors. Since it is a cytotoxic agent, bleomycin is known to induce endothelial damage the lung vasculature referred to as bleomycin-induced lung-injury or bleomycin-induced pneumonitis (BIP). Researchers have used this property of bleomycin to induce lung fibrosis in mouse and rat models.


15 units in serum bottle

Biochem/physiol Actions

Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.


This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.


This product should be stored at 2-8°C.

Preparation Note

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.

Other Notes

Keep container tightly closed in a dry and well-ventilated place, strongly hygroscopic


Health hazard

Signal Word


Hazard Statements

Hazard Classifications

Carc. 2 - Muta. 1B - Repr. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Donald H Atha et al.
Journal of nucleic acids, 2020, 8810105-8810105 (2020-08-18)
To evaluate methods for analysis of genotoxic effects on mammalian cell lines, we tested the effect of three common genotoxic agents on Chinese hamster ovary (CHO) cells by single-cell gel electrophoresis (comet assay) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Suspension-grown
Markus Berger et al.
Journal of medicinal chemistry, 64(17), 12723-12737 (2021-08-25)
Eukaryotes have evolved two major pathways to repair potentially lethal DNA double-strand breaks. Homologous recombination represents a precise, DNA-template-based mechanism available during the S and G2 cell cycle phase, whereas non-homologous end joining, which requires DNA-dependent protein kinase (DNA-PK), allows
Andrea M Foskett et al.
American journal of physiology. Lung cellular and molecular physiology, 306(2), L120-L131 (2013-11-19)
Previous reports demonstrated that bleomycin-induced injury of lungs in mice can be improved by the administration of murine multipotent adult stem/progenitor cells (MSCs) from the bone marrow. Recently some of the beneficial effects of MSCs have been explained by the
Andrea C Lo et al.
International journal of radiation oncology, biology, physics, 88(5), 1011-1018 (2014-03-26)
We report long-term outcomes and complications of craniopharyngioma patients referred to our institution. Between 1971 and 2010, 123 consecutive patients received primary treatment for craniopharyngioma in British Columbia and were referred to our institution. The median age was 30 years
Britta Maurer et al.
Annals of the rheumatic diseases, 72(12), 2039-2046 (2013-09-10)
To assess whether the discrepancy between the strong antifibrotic effects of tyrosine kinase inhibitors (TKIs) in animal models and the inconsistent results in clinical studies might be related to the activation levels of drug targets. Skin sections of bleomycin, TSK1


Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

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