B8781

Restriction Enzyme Buffer SB

Name: Restriction Enzyme Buffer SB
Brand: SIGMA
Price: 64.7 USD

10 ×

Synonym(s):

Restriction Endonuclease Buffer

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About This Item

NACRES:

NA.53

UNSPSC Code:

12352204

Key Documents

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InChI

1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)

InChI key

YNZJHLFHFNCZIE-UHFFFAOYSA-N

SMILES string

CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C

grade

Molecular Biology

description

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl₂, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

form

buffered aqueous solution

concentration

10 ×

storage temp.

−20°C

Quality Level

200

Related Categories

Biochemicals

Biological Buffers

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1 of 4

This Item	S6639	S2015	B6429
Sigma-Aldrich B8781 Restriction Enzyme Buffer SB	Sigma-Aldrich S6639 SSC Buffer 20× Concentrate	Sigma-Aldrich S2015 SSPE Buffer 20× Concentrate	Sigma-Aldrich B6429 Casein Blocking Buffer 10x
grade for molecular biology	grade for molecular biology	grade for molecular biology	grade for molecular biology
concentration 10 ×	concentration -	concentration -	concentration -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form buffered aqueous solution	form solution	form solution	form solution
storage temp. −20°C	storage temp. room temp	storage temp. room temp	storage temp. room temp
description 10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl₂, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.	description Saline-Sodium Citrate buffer, made with ultrapure water	description -	description -

General description

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

Application

Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.[1]

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+ C nucleotide content and secondary structure melting point

Bukovska P, et al.

Journal of Microbiological Methods, 82(3), 223-228 (2010)

Articles

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

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Restriction Enzyme Buffer SB