B8894

Sigma-Aldrich

Albumin, Acetylated from bovine serum

protease free, for molecular biology

Synonym(s):
Ac-BSA, Acetyl BSA, Acetylated BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

grade

for molecular biology

product line

BioReagent

form

solution

purified by

heat shock fractionation

contains

does not contain stabilizer or preservative

origin

USA origin

concentration

20 mg/mL in H2O

application(s)

FISH: suitable

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

UniProt accession no.

foreign activity

DNase free
DNase, RNase, NICKase and protease, none detected
NICKase free
Protease free
RNase free

storage temp.

−20°C

Gene Information

bovine ... ALB(280717)

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General description

Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.

Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.

Application

Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:
  • In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
  • As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Biochem/physiol Actions

Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.

Features and Benefits

This acetylated BSA product has been tested for the following properties:
  • DNase-free / Exonuclease-free
  • Nickase-free / Endonuclease-free
  • Protease-free
  • RNase-free

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Ivan Haralampiev et al.
Nature communications, 11(1), 4355-4355 (2020-08-30)
The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are...
Fan Guo et al.
Cell research, 27(8), 967-988 (2017-06-18)
Single-cell epigenome sequencing techniques have recently been developed. However, the combination of different layers of epigenome sequencing in an individual cell has not yet been achieved. Here, we developed a single-cell multi-omics sequencing technology (single-cell COOL-seq) that can analyze the...
Markus Hafner et al.
Methods (San Diego, Calif.), 44(1), 3-12 (2007-12-26)
Distinct classes of small RNAs, 20-32 nucleotides long, play important regulatory roles for diverse cellular processes. It is therefore important to identify and quantify small RNAs as a function of development, tissue and cell type, in normal and disease states....
Bill X Huang et al.
Journal of the American Society for Mass Spectrometry, 15(8), 1237-1247 (2004-07-28)
Serum albumin is the principal transporter of fatty acids that are otherwise insoluble in circulating plasma. While the crystal structure of human serum albumin (HSA) as well as its binding with fatty acids has been characterized, the three dimensional structure...
Francesca Rossiello et al.
Nature communications, 8, 13980-13980 (2017-02-28)
The DNA damage response (DDR) is a set of cellular events that follows the generation of DNA damage. Recently, site-specific small non-coding RNAs, also termed DNA damage response RNAs (DDRNAs), have been shown to play a role in DDR signalling...

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