MilliporeSigma
All Photos(2)

B8894

Sigma-Aldrich

Albumin, Acetylated from bovine serum

protease free, for molecular biology

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
Ac-BSA, Acetyl BSA, Acetylated BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

grade

for molecular biology

product line

BioReagent

form

solution

purified by

heat shock fractionation

contains

does not contain stabilizer or preservative

origin

USA origin

concentration

20 mg/mL in H2O

technique(s)

FISH: suitable

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

UniProt accession no.

foreign activity

DNase free
DNase, RNase, NICKase and protease, none detected
NICKase free
Protease free
RNase free

storage temp.

−20°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
B2518B8667A0281
Bovine Serum Albumin lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis)

Sigma-Aldrich

A0281

Bovine Serum Albumin

grade

for molecular biology

grade

-

grade

for molecular biology

grade

-

form

solution

form

lyophilized powder

form

solution

form

lyophilized powder

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

cold ethanol and heatshock fractionation

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

concentration

20 mg/mL in H2O

concentration

-

concentration

20 mg/mL in H2O, 20-22 mg/mL Biuret

concentration

-

General description

Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.

Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.

Application

Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:
  • In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
  • As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Biochem/physiol Actions

Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.

Features and Benefits

This acetylated BSA product has been tested for the following properties:
  • DNase-free / Exonuclease-free
  • Nickase-free / Endonuclease-free
  • Protease-free
  • RNase-free

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Bovine Serum Albumin lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

A9418

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, Protease, essentially free, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A3858

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, suitable for RIA, pH 5.2, ≥96%

Sigma-Aldrich

A7888

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, crystallized, ≥98.0% (GE)

Sigma-Aldrich

05470

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A2153

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, low endotoxin, pH 7, ≥98%

Sigma-Aldrich

A9543

Bovine Serum Albumin

Xiang Yu et al.
STAR protocols, 2(4), 100901-100901 (2021-11-25)
Eukaryotic RNAs can be modified with a non-canonical 5' nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not
Fan Guo et al.
Cell research, 27(8), 967-988 (2017-06-18)
Single-cell epigenome sequencing techniques have recently been developed. However, the combination of different layers of epigenome sequencing in an individual cell has not yet been achieved. Here, we developed a single-cell multi-omics sequencing technology (single-cell COOL-seq) that can analyze the
Ivan Haralampiev et al.
Nature communications, 11(1), 4355-4355 (2020-08-30)
The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are
Hongshan Guo et al.
Nature protocols, 10(5), 645-659 (2015-04-04)
The heterogeneity of DNA methylation within a population of cells necessitates DNA methylome profiling at single-cell resolution. Recently, we developed a single-cell reduced-representation bisulfite sequencing (scRRBS) technique in which we modified the original RRBS method by integrating all the experimental
Catherine Chailleux et al.
Nature protocols, 9(3), 517-528 (2014-02-08)
Recent advances in our understanding of the management and repair of DNA double-strand breaks (DSBs) rely on the study of targeted DSBs that have been induced in living cells by the controlled activity of site-specific endonucleases, usually recombinant restriction enzymes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service