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MilliporeSigma

B8931

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):

Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside

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25 MG
$241.00

$241.00

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
93863-88-8
Molecular Weight:
408.63
MDL number:
MFCD02683897
UNSPSC Code:
12352200
PubChem Substance ID:
329773145
NACRES:
NA.85

Key Documents

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grade

Molecular Biology

Quality Level

200

sterility

non-sterile

product line

BioReagent

assay

≥98.0% (HPLC)

form

powder

solubility

methanol: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1

InChI key

CHRVKCMQIZYLNM-HTOAHKCRSA-N

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1 of 4

This Item
S9811B2904RES1364C
Magenta-Gal reagent for selection of recombinant bacterial clones

Sigma-Aldrich

B8931

Magenta-Gal

S-Gal® reagent for selection of recombinant bacterial clones

Sigma-Aldrich

S9811

S-Gal®

Bluo-Gal reagent for selection of recombinant bacterial clones

Sigma-Aldrich

B2904

Bluo-Gal

Red-Gal®

Sigma-Aldrich

RES1364C

Red-Gal®

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

-

suitability

suitable for substrate for β-galactosidase

suitability

suitable for β-galactosidase test

suitability

suitable for substrate for β-galactosidase

suitability

-

solubility

methanol: soluble

solubility

DMF: 50 mg/mL

solubility

DMF: soluble

solubility

-

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

300

General description

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase,[1] used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA.[2] It has also been used to detect the expression of the EphB1-β-gal fusion protein.[1] ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.

Biochem/physiol Actions

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ9184
BCBK8608V
BCBJ5700V
1403239V
1403239

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Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos
In Situ Hybridization Methods, 151-167 (2015)
Susan C McCutcheon et al.
BMC biology, 8, 89-89 (2010-06-24)
Reporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum
George Chenaux et al.
The European journal of neuroscience, 34(10), 1620-1633 (2011-11-23)
EphB receptor tyrosine kinases direct axonal pathfinding through interactions with ephrin-B proteins following axon-cell contact. As EphB:ephrin-B binding leads to bidirectional signals, the contributions of signaling into the Eph-expressing cell (forward signaling) or the ephrin-expressing cell (reverse signaling) cannot be
Maria Belen Jaurena et al.
Nature communications, 6, 7476-7476 (2015-06-24)
All cranial placode progenitors arise from a common precursor field anterior to the neural plate, the pre-placodal region (PPR). We showed that transcription factor Zic1, expressed at the anterior neural plate, is necessary and sufficient to promote placode fate. Here
Yeon-Jin Kim et al.
Developmental biology, 397(1), 129-139 (2014-12-03)
Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and

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