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B9433

Sigma-Aldrich

Bovine Serum, Adult

USA origin, sterile-filtered, suitable for cell culture

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Synonym(s):
Bovine sera, cow sera, cow serum
eCl@ss:
32160409
NACRES:
NA.71

biological source

bovine

Quality Level

500

sterility

sterile-filtered

form

liquid

composition

hemoglobin, ≤50 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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B9433

Bovine Serum, Adult

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12133C

Bovine Calf Serum

Fetal Bovine Serum Australia origin, USDA approved, Heat Inactivated, suitable for cell culture

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Fetal Bovine Serum

Fetal Bovine Serum Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

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F8192

Fetal Bovine Serum

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

-

form

-

form

-

composition

hemoglobin, ≤50 mg/dL

composition

hemoglobin, ≤35 mg/dL

composition

Hemoglobin, ≤25 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

origin

USA origin

origin

Australia origin

origin

Australia origin

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

General description

Bovine serum (BS) is considered a cost-effective choice than fetal bovine serum (FBS). It can also be preserved stable for a long time.

Application

Bovine Serum, Adult has been used:
  • to treat silicone square to prepare it for the biofilm assay for in vitro biofilm growth conditions
  • in cell culture as a component in Dulbecco′s modified Eagle medium for digesting articular cartilage and proximal metacarpals
  • as a lubricating solution in hydrodynamic analysis and to imitate the synovial joints

Biochem/physiol Actions

Adult bovine serum (BS) possesses bactericidal activity against Helicobacter pylori. Heat-treated BS can be used as the best serum source of H. pylori culture medium.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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In vitro cellular & developmental biology. Animal, 30A(5), 295-299 (1994-05-01)
A mixture containing an ultrafiltrate fraction (UF) of bovine colostrum (6.7%), adult bovine serum (BS) (1%), and human holo-transferrin (hTF) (5 mg/liter) was developed for cultivation of Chinese hamster ovary cells (CHO-K1) and African green monkey kidney cells (Vero). The
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Cells are often cultured at high density (e.g., confluent monolayer and as pellets) to promote chondrogenic differentiation and to maintain the chondrocyte phenotype. They are also frequently suspended in hydrogels such as agarose or alginate for the same purposes. These
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