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B9754

Sigma-Aldrich

BIS-TRIS

≥98.0% (titration)

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Synonym(s):
2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
6976-37-0
Molecular Weight:
209.24
Beilstein:
2205275
EC Number:
230-237-7
MDL number:
MFCD00002853
PubChem Substance ID:
24892155
NACRES:
NA.25

Quality Level

400

Assay

≥98.0% (titration)

form

crystalline powder

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

application(s)

diagnostic assay manufacturing

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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1 of 4

This Item
B4429B753514879
BIS-TRIS ≥98.0% (titration)

Sigma-Aldrich

B9754

BIS-TRIS

BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

Sigma-Aldrich

B4429

BIS-TRIS

BIS-TRIS BioXtra, ≥98.0% (titration)

Sigma-Aldrich

B7535

BIS-TRIS

BIS-TRIS BioUltra, ≥99.0% (NT)

Sigma-Aldrich

14879

BIS-TRIS

form

crystalline powder

form

crystalline powder

form

powder

form

powder

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 1 M, clear, colorless

solubility

H2O: 1 M at 20 °C, clear, colorless

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

200

useful pH range

5.8-7.2

useful pH range

5.8-7.2

useful pH range

5.8-7.2

useful pH range

5.8-7.2

General description

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

Application

Bis-Tris has been used :
  • as a buffer for Electrophoresis
  • as a component of transfer buffer for Western Blotting

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Other Notes

Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318

Precautionary Statements

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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2,2-Bis(hydroxyethyl)-(iminotris)- (hydroxymethyl)-methane BIS-TRIS

Millipore

1.03252

2,2-Bis(hydroxyethyl)-(iminotris)- (hydroxymethyl)-methane

BIS-Tris, ULTROL® Grade A zwitterionic buffer that exhibits only a very small shift in dissociation constant with respect to temperature.

Millipore

391335

BIS-Tris, ULTROL® Grade

MOPSO ≥99% (titration)

Sigma-Aldrich

M8389

MOPSO

Tricine ≥99% (titration)

Sigma-Aldrich

T0377

Tricine

BIS-TRIS hydrochloride

SAFC

RES1164B-A7

BIS-TRIS hydrochloride

MES hydrate ≥99.5% (titration)

Sigma-Aldrich

M8250

MES hydrate

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