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B9804

Sigma-Aldrich

Anti-Bcl-2 antibody, Mouse monoclonal

clone 10C4, purified from hybridoma cell culture

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10C4, monoclonal

form

buffered aqueous solution

mol wt

antigen 26 kDa

species reactivity

rat, mouse

concentration

~2 mg/mL

application(s)

microarray: suitable
western blot: 2-10 μg/mL using rat osteosarcoma ROS cell extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Bcl2(12043)
rat ... Bcl2(24224)

Specificity

The epitope recognized by the antibody resides within amino acids 61-76 of the mouse Bcl-2 protein.

Immunogen

synthetic peptide corresponding to amino acids 61-76 of the mouse Bcl-2 sequence, conjugated to KLH.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Beclin 1 Interacts With Active Caspase-3 and Bax in Oocytes From Atretic Follicles in the Rat Ovary.
Escobar, et al.
The Journal of Histochemistry and Cytochemistry, 67, 873-889 (2020)
Zhen-Xing Huang et al.
Brain research, 1153, 204-213 (2007-04-28)
It has been established that hyperbaric oxygen (HBO) treatment reduces brain edema, decreases infarct volume, contributes to neurological functional recovery and suppresses apoptosis in suture-induced focal cerebral ischemic animal models. In the present study, we evaluated the therapeutic effect of...
Y T Hsu et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(8), 3668-3672 (1997-04-15)
Bcl-2, Bcl-X(L), and Bax are members of the Bcl-2 family that play key roles in the regulation of apoptosis. These proteins are believed to be membrane bound and their ability to undergo both homodimerization and heterodimerization has been proposed to...
Wenfei Zhao et al.
Oncology letters, 18(5), 4613-4620 (2019-10-16)
The present study aimed to investigate the association between microRNA-152 and cisplatin resistance in non-small cell lung cancer. A549 and cisplatin-resistant A549 cells (A549/cis) were maintained in vitro. Reverse transcription-quantitative PCR (RT-qPCR) was performed to analyze differences in microRNA-152 levels...
Elena Piegari et al.
Scientific reports, 10(1), 12250-12250 (2020-07-25)
Cardiotoxicity remains a serious problem in anthracycline-treated oncologic patients. Therapeutic modulation of microRNA expression is emerging as a cardioprotective approach in several cardiovascular pathologies. MiR-34a increased in animals and patients exposed to anthracyclines and is involved in cardiac repair. In...

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