BCK-EDU488

ClickTech EdU Cell Proliferation Kit 488 for IM

Name: ClickTech EdU Cell Proliferation Kit 488 for IM
Brand: SIGMA
Price: 611 USD

sufficient for 100 assays

Synonym(s):

EdU Cell Proliferation Kit

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About This Item

NACRES:

NA.22

UNSPSC Code:

12161503

Key Documents

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fluorescence

λ_ex 496 nm; λ_em 516 nm

shipped in

wet ice

storage temp.

2-8°C

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This Item	BCK-EDU594	BCK-EDU647	BCK-EDU555
Sigma-Aldrich BCK-EDU488 ClickTech EdU Cell Proliferation Kit 488 for IM	Sigma-Aldrich BCK-EDU594 ClickTech EdU Cell Proliferation Kit 594 for IM	Sigma-Aldrich BCK-EDU647 ClickTech EdU Cell Proliferation Kit 647 for IM	Sigma-Aldrich BCK-EDU555 ClickTech EdU Cell Proliferation Kit 555 for IM
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
fluorescence λ_ex 496 nm; λ_em 516 nm	fluorescence λ_ex 584 nm; λ_em 603 nm	fluorescence λ_ex 641 nm; λ_em 663 nm	fluorescence λ_ex 546 nm; λ_em 579 nm

General description

EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the Baseclick EdU-Click Assays utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Application

Imaging

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H319,H340,H361,H412

pcodes

P202 - P264 - P273 - P280 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation.

Drake S Edwards et al.

Cell reports, 32(12), 108166-108166 (2020-09-24)

Effective spatio-temporal control of transcription and replication during S-phase is paramount to maintaining genomic integrity and cell survival. Dysregulation of these systems can lead to conflicts between the transcription and replication machinery, causing DNA damage and cell death. BRD4 allows

Isolation, culture, and characterization of chicken intestinal epithelial cells.

Federico Ghiselli et al.

BMC molecular and cell biology, 22(1), 12-12 (2021-02-14)

Enterocytes exert an absorptive and protective function in the intestine, and they encounter many different challenging factors such as feed, bacteria, and parasites. An intestinal epithelial in vitro model can help to understand how enterocytes are affected by these factors

EdU incorporation is an alternative non-radioactive assay to [(3)H]thymidine uptake for in vitro measurement of mice T-cell proliferations.

Yongmao Yu et al.

Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)

T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the

EdU, a new thymidine analogue for labelling proliferating cells in the nervous system.

Fatemah Chehrehasa et al.

Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)

Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

Articles

Cell Proliferation EdU Assay for DNA Synthesis Detection

Explore the EdU Cell Proliferation Assay for fast DNA synthesis detection in cell biology, oncology, and drug research.

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