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BCK-EDU488

Sigma-Aldrich

EdU-Click 488

sufficient for 100 assays

All Photos(1)
NACRES:
NA.22

fluorescence

λex 496 nm; λem 516 nm

shipped in

wet ice

storage temp.

2-8°C

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BCK-EDU555BCK-EDU594BCK-HTS488-2
EdU-Click 488 sufficient for 100 assays

Sigma-Aldrich

BCK-EDU488

EdU-Click 488

EdU-Click 555 sufficient for 100 assays

Sigma-Aldrich

BCK-EDU555

EdU-Click 555

EdU-Click 594 sufficient for 100 assays

Sigma-Aldrich

BCK-EDU594

EdU-Click 594

EdU HTS Kit 488 sufficient for 200 assays

Sigma-Aldrich

BCK-HTS488-2

EdU HTS Kit 488

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

General description

EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the Baseclick EdU-Click Assays utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Application

Imaging

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H319 - H340 - H361 - H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

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Drake S Edwards et al.
Cell reports, 32(12), 108166-108166 (2020-09-24)
Effective spatio-temporal control of transcription and replication during S-phase is paramount to maintaining genomic integrity and cell survival. Dysregulation of these systems can lead to conflicts between the transcription and replication machinery, causing DNA damage and cell death. BRD4 allows
Federico Ghiselli et al.
BMC molecular and cell biology, 22(1), 12-12 (2021-02-14)
Enterocytes exert an absorptive and protective function in the intestine, and they encounter many different challenging factors such as feed, bacteria, and parasites. An intestinal epithelial in vitro model can help to understand how enterocytes are affected by these factors
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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