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C0356

Sigma-Aldrich

Protein Extraction Reagent Type 4

NACRES:
NA.56

solubility

deionized water: 13.6 g /15 ml at 25 °C, clear to hazy

Quality Level

General description

Protein Extraction Reagent Type 4, is a highly premixed, prequalified and effective protein solubilization and extraction reagent composed of urea, thiourea, Trizma®, and detergent C7BzO (C0856).

Application

Protein extraction reagent type 4 is optimized for proteomic studies. Cellular and organelle membrane solubilizing reagent is ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. Reducing and alkylating reagents TBP (T7567) and iodoacetamide (A3221) are recommended for use with this reagent. Protein extraction reagent type 4 is also available as a component of the ProteoPrep® Sample.

Packaging

4 bottles in poly bottle

Components

Contains urea, thiourea, Trizma®, and detergent C7BzO (C0856).

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Roger A Moore et al.
Proteomics, 10(15), 2858-2869 (2010-06-03)
Transmissible spongiform encephalopathies (TSEs) or prion diseases are characterized by the accumulation of an aggregated isoform of the prion protein (PrP). This pathological isoform, termed PrP(Sc), appears to be the primary component of the TSE infectious agent or prion. However
Gabriel A Grilo et al.
Journal of molecular and cellular cardiology, 139, 62-74 (2020-01-25)
Age-related remodeling of the heart causes structural and functional changes in the left ventricle (LV) that are associated with a high index of morbidities and mortality worldwide. Some cardiac pathologies in the elderly population vary between genders revealing that cardiac
Meritxell Pérez-Hedo et al.
Proteome science, 10(1), 58-58 (2012-10-02)
Sesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared under short day conditions enter
John McCarthy et al.
Journal of proteome research, 2(3), 239-242 (2003-06-20)
Carbamylation is widely quoted as being a problem in 2-D gel analysis and the associated sample preparation steps. This modification occurs when iso-cyanate, a urea break-down product, covalently modifies lysine residues, thus inducing a change in isoelectric point. Urea is
Chawalit Kocharunchitt et al.
Molecular & cellular proteomics : MCP, 11(1), M111-M111 (2011-10-20)
An integrated transcriptomic and proteomic analysis was undertaken to determine the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcass chilling in cold air. The response of

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