C0733

Cyclooxygenase 1 from sheep

glycerol solution, ≥1500 units/mg protein

• Synonym(s): COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase
• Enzyme Commission number: 1.14.99.1 (BRENDA, IUBMB)
• MDL number: MFCD02098124
• NACRES: NA.32

Properties

• form: glycerol solution
• Quality Level: 200
• specific activity: ≥1500 units/mg protein
• mol wt: dimer subunit mol wt 70 kDa
• UniProt accession no.: O78749
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: sheep ... COX1(808251)

Description

General description

Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain.[1] Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.[2]

Application

Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples[3] and in prostaglandin synthase activity assay.[4]

Biochem/physiol Actions

COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H₂ (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent K_M of 8.3 μM for arachidonic acid.

Unit Definition

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

Physical form

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN^® 20, 300 μM diethyldithiocarbamate and 10% glycerol.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable