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C0733

Sigma-Aldrich

Cyclooxygenase 1 from sheep

glycerol solution, ≥1500 units/mg protein

Synonym(s):
COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase
Enzyme Commission number:
MDL number:
NACRES:
NA.32

form

glycerol solution

Quality Level

specific activity

≥1500 units/mg protein

mol wt

dimer subunit mol wt 70 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

sheep ... COX1(808251)

General description

Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain.[1] Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.[2]

Application

Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples[3] and in prostaglandin synthase activity assay.[4]

Biochem/physiol Actions

COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent KM of 8.3 μM for arachidonic acid.

Unit Definition

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

Physical form

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN® 20, 300 μM diethyldithiocarbamate and 10% glycerol.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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