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C0775

Sigma-Aldrich

Crystal Violet

certified by the Biological Stain Commission

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Synonym(s):
Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
MDL number:
PubChem Substance ID:

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥90%

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

1.19 g/cm3 at 20 °C

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

Mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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This Item
C615861135G2039
Crystal Violet certified by the Biological Stain Commission

Sigma-Aldrich

C0775

Crystal Violet

Crystal Violet ACS reagent, ≥90.0% anhydrous basis

Sigma-Aldrich

C6158

Crystal Violet

Crystal Violet for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

Sigma-Aldrich

61135

Crystal Violet

Gentian Violet meets USP testing specifications

Sigma-Aldrich

G2039

Gentian Violet

form

powder

form

powder

form

powder

form

-

composition

Dye content, ≥90%

composition

-

composition

-

composition

-

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

solubility

water: soluble 50 g/L at 27 °C

solubility

-

solubility

-

density

1.19 g/cm3 at 20 °C

density

1.19 g/cm3 at 20 °C

density

1.190 g/cm3

density

-

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Suitability

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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USP

1290002

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Sigma-Aldrich

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Lipocalin 2 induces the epithelial-mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model.
Liao CJ, et al.
Reproduction (Cambridge, England), 147, 179-179 (2013)
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
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