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C0775

Sigma-Aldrich

Crystal Violet

certified by the Biological Stain Commission

Synonym(s):
Hexamethylpararosaniline chloride, Methyl Violet 10B, Basic Violet 3, Gentian Violet
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥90%

application(s)

hematology: suitable
histology: suitable

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

1.19 g/cm3 at 20 °C

Featured Industry

Diagnostic Assay Manufacturing

Mode of action

cell membrane | interferes
enzyme | inhibits

antibiotic activity spectrum

fungi

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Suitability

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Lipocalin 2 induces the epithelial-mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model.
Liao CJ, et al.
Reproduction (Cambridge, England), 147, 179-179 (2013)
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)
Vera van de Pol et al.
International journal of molecular sciences, 20(13) (2019-07-05)
Bicuspid aortic valve (BAV), the most common congenital heart defect, is associated with an increased prevalence of aortic dilation, aortic rupture and aortic valve calcification. Endothelial cells (ECs) play a major role in vessel wall integrity. Little is known regarding...
Carson S and Robertson D
Manipulation and Expression of Recombinant DNA (2005)
Ki Hyun Kim et al.
PLoS pathogens, 15(2), e1007375-e1007375 (2019-02-02)
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease localized to China, Japan, and Korea that is characterized by severe hemorrhage and a high fatality rate. Currently, no specific vaccine or treatment has been approved for this disease....

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