Perfect for isolating colonies in cell culture under sterile conditions. The adhesive grease doesn't affect the cells and the plate from which colonies are derived can be left for further cell growth. Easy and straightforward protocol. Used 75uL Trypsin for dissociation and 150uL of growth medium for re-suspension. Was able to obtain and expand clones for analysis.
C1059
Cloning cylinders, glass
volume 150 μL
Cloning cylinders, glass
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Synonym(s):
cloning cylinder, cloning equipment
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1 EA
$78.80
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About This Item
UNSPSC Code:
41106618
NACRES:
NB.22
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material
light brown (tinted)
sterility
sterile; electron beam irradiated
packaging
dish of 15 cylinders
manufacturer/tradename
Millipore TR1004
size
8 mm × 8 mm
volume
150 μL
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Related Categories
1 of 4
This Item | C3858 | C7983 | C3983 |
|---|---|---|---|
| sterility sterile; electron beam irradiated | sterility sterile | sterility sterile | sterility sterile |
| material light brown (tinted) | material - | material - | material - |
| manufacturer/tradename Millipore TR1004 | manufacturer/tradename Bel-Art F37847-0300 | manufacturer/tradename Bel-Art F37847-0100 | manufacturer/tradename Bel-Art F37847-0200 |
| packaging dish of 15 cylinders | packaging pack of 50 ea | packaging pack of 50 ea | packaging pack of 50 ea |
| size 8 mm × 8 mm | size - | size - | size - |
| volume 150 μL | volume - | volume - | volume - |
General description
Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.
Packaging
Packaged in 60 mm dish with silicone grease on one end
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Tamara D Kolokoltsova et al.
Human cell, 30(4), 249-257 (2017-06-15)
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D Geerts et al.
The Journal of cell biology, 147(2), 417-434 (1999-10-20)
Hemidesmosomes are stable adhesion complexes in basal epithelial cells that provide a link between the intermediate filament network and the extracellular matrix. We have investigated the recruitment of plectin into hemidesmosomes by the alpha6beta4 integrin and have shown that the
Maria Chiara Lionetti et al.
Biophysical journal, 118(9), 2319-2332 (2020-04-23)
The nuclear morphology of eukaryotic cells is determined by the interplay between the lamina forming the nuclear skeleton, the chromatin inside the nucleus, and the coupling with the cytoskeleton. Nuclear alterations are often associated with pathological conditions as in Hutchinson-Gilford
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