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C1224

Caspase 3 human

≥85% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym(s):

Apopain, CPP32, Yama

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10 μG

$711.00

$711.00

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
3.4.22.56 (BRENDA, IUBMB)
MDL number:
MFCD01634593
Specific activity:
≥1.0 units/mg protein
Assay:
≥85% (SDS-PAGE)
Recombinant:
expressed in E. coli (C-terminal histidine-tagged)

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recombinant

expressed in E. coli (C-terminal histidine-tagged)

assay

≥85% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥1.0 units/mg protein

mol wt

~30 kDa

UniProt accession no.

P42574

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

human ... CASP3(836)

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This Item
AB3623C9598C8726
Caspase 3 human ≥85% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

Sigma-Aldrich

C1224

Caspase 3 human

Anti-Caspase 3 Antibody, active (cleaved) form Chemicon®, from rabbit

Sigma-Aldrich

AB3623

Anti-Caspase 3 Antibody, active (cleaved) form

Anti-Caspase 3 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

C9598

Anti-Caspase 3 antibody produced in rabbit

Caspase 9 human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous solution, >2,000 units/mg protein

Sigma-Aldrich

C8726

Caspase 9 human

specific activity

≥1.0 units/mg protein

specific activity

-

specific activity

-

specific activity

>2,000 units/mg protein

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP9(842)

assay

≥85% (SDS-PAGE)

assay

-

assay

-

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

recombinant

expressed in E. coli (C-terminal histidine-tagged)

recombinant

-

recombinant

-

recombinant

expressed in E. coli (C-terminal histidine-tagged)

mol wt

~30 kDa

mol wt

-

mol wt

antigen 32 kDa

mol wt

N-terminal prodomain plus the large subunit. 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein), small subunit 13 kDa (subunit containing the histidine tag)

Application

Caspase 3 human has been used to digest human r-vimentin.[1] It has also been used in caspase-3 inhibition assay[2] and protease inhibition assay.[3]

This product may also be used in cell-based apoptosis assay.

Biochem/physiol Actions

Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.
Member of the CED-3 subfamily of caspases and responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation.

General description

Research area: Apoptosis

Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.

Other Notes

One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.

Physical form

Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS

Preparation Note

Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000359506
0000359506
086M4074V
076M4150V
045M4099V

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Discovery of indole tetrafluorophenoxymethylketone-based potent novel small molecule inhibitors of caspase-3
Samiulla DS, et al.
Organic and Medicinal Chemistry Letters, 2(1), 27-27 (2012)
Deju Ye et al.
Nature chemistry, 6(6), 519-526 (2014-05-23)
Directed self-assembly of small molecules in living systems could enable a myriad of applications in biology and medicine, and already this has been used widely to synthesize supramolecules and nano/microstructures in solution and in living cells. However, controlling the self-assembly
Regulation of spermatogenic cell apoptosis by the pro-apoptotic proteins in the testicular tissues of mammalian and avian species
Zakariah M, et al.
Animal Reproduction Science (2022)
Petra Procházková et al.
PloS one, 9(10), e109900-e109900 (2014-10-04)
Iron homeostasis in cells is regulated by iron regulatory proteins (IRPs) that exist in different organisms. IRPs are cytosolic proteins that bind to iron-responsive elements (IREs) of the 5'- or 3'-untranslated regions (UTR) of mRNAs that encode many proteins involved
Cross-presentation of caspase-cleaved apoptotic self antigens in HIV infection
Rawson PM, et al.
Nature Medicine, 13(12), 1431-1431 (2007)
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