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C-12710

Sigma-Aldrich

Human Chondrocytes (HCH)

500,000 cryopreserved cells

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biological source

human articular cartilage (knee and hip joints)

packaging

pkg of 500,000 cells

morphology

(chondrocytes)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12901C-14080C-14036
packaging

pkg of 500,000 cells

packaging

pkg of 25,000,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 1,000,000 cells

morphology

(chondrocytes)

morphology

(mononuclear)

morphology

(myocytes)

morphology

(fibroblast)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−20°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Cartilage

Application

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request) and are provided in a cryopreserved format. HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Quality

Rigid quality control tests are performed for each lot of Human Chondrocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, each lot is routinely tested for the capacity to differentiate in 3D spheroids. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV and HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Differentiation testedGuaranteed population doublings: >10

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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