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C-12810

Sigma-Aldrich

Human Cardiac Myocytes (HCM)

500,000 cryopreserved cells

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Synonym(s):
HCM cells

biological source

human heart (ventricles)

packaging

pkg of 500,000 cells

morphology

(myocytes)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12375C-14080C-12907
vibrant-m

C-12810

Human Cardiac Myocytes (HCM)

vibrant-m

C-12375

Human Cardiac Fibroblasts (HCF)

vibrant-m

C-14080

Human Cardiac Myocytes (HCM)

vibrant-m

C-12907

Human Mononuclear Cells (hMNC)

biological source

human heart (ventricles)

biological source

human heart (ventricles)

biological source

human heart (ventricles)

biological source

human peripheral blood

morphology

(myocytes)

morphology

(fibroblast)

morphology

(myocytes)

morphology

(mononuclear)

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−196°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 25,000,000 cells

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Heart

Application

Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart and are provided in a cryopreserved format. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers of early stage differentiation such as GATA-4 and sarcomeric alpha-actin and have a high capacity for proliferation. When they are grown to confluency and cultivated for an extended period of time, the differentiation process begins. Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to form myotube-like structures.

Quality

Rigid quality control tests are performed for each lot of Human Cardiac Myocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, flow cytometric analyses for cell-type specific markers, e.g. sarcomeric a-actinin and slow muscle myosin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Sarcomeric alpha-actinin positive; Slow muscle myosin positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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