C-12916

Human M1 Macrophages (hMDM-GMCSF(-))

Monocyte-derived, single donor, 5 million cryopreserved cells

• Synonym(s): hMDM-GMCSF(-) cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human blood (monocyte)
• Morphology: (macrophages)

Properties

• biological source: human blood (monocyte)
• packaging: pkg of 500,000 cells
• morphology: (macrophages)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Application

Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M1 Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 5 million viable cells after thawing.

Biochem/physiol Actions

Monocyte

Preparation Note

Click here for more information.

Other Notes

Recommended Plating Density: 100000 cells per cm²Tested Markers: CD80 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.

Recommended Primary Cell Culture Media:Link

Recommended Primary Cell Culture Media:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable