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C1399

Sigma-Aldrich

Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse

clone KS-B17.2, ascites fluid

Synonym(s):

Anti-CK18

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

KS-B17.2, monoclonal

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human tissue
microarray: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KRT18(3875)

General description

Monoclonal anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

The antibody reacts with a wide variety of simple epithelia (gastrointestinal, respiratory, urinary, liver, and glandular) but does not react with most stratified squamous epithelium (esophagus, epidermis) or non-epithelial cells.

Immunogen

keratin from the bovine mammary gland epithelial cell line BMGE.

Application

Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse has been used in:
  • immunofluorescence
  • immunoperoxidase staining
  • immunocytochemistry
  • microarray

Biochem/physiol Actions

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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S K Mohapatra et al.
Animal : an international journal of animal bioscience, 9(4), 643-649 (2014-12-11)
Insulin-like growth factor binding protein-5 (IGFBP-5) is a key molecule in mammary gland development, which facilitates the removal of mammary epithelial cells (MECs) by apoptosis that takes place during remodeling of the mammary gland during involution. IGFBP-5 binds with IGFs
Trehalose maintains vitality of mouse epididymal epithelial cells and mediates gene transfer.
Qu B, et al.
PLoS ONE, 9(3), e92483-e92483 (2014)
P H Chen et al.
Journal of virology, 67(6), 3507-3514 (1993-06-01)
Immunofluorescence studies revealed that adenovirus induces a reorganization of the cytokeratin system in lytically infected HeLa cells. At 24 h postinfection, the cytokeratin network began to disassemble into prominent spheroid globules. By 36 h postinfection, host cell lysis occurred, accompanied
The identification and localization of two intermediate filament proteins in the tunic of Styela plicata (Tunicata, Styelidae).
Di Bella MA, et al.
Tissue & cell, 41(6), 381-389 (2009)
M J Wells et al.
The Journal of biological chemistry, 272(45), 28574-28581 (1997-11-14)
During experiments to identify putative hepatic receptors for thrombin-antithrombin (TAT) complexes, a 45-kDa protein was identified by ligand blotting. Following gel purification, amino acid sequencing revealed the 45-kDa TAT-binding polypeptide to be cytokeratin 18 (CK18). The presence of CK18 on

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