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C1403

Sigma-Aldrich

Cholesterol Esterase from Pseudomonas sp.

lyophilized powder, ≥200,000 units/g protein

Synonym(s):

Cholesterol Esterase from Pseudomonas fluorescens, Sterol-ester acylhydrolase

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About This Item

CAS Number:
9026-00-0
EC Number:
3.1.1.13 (BRENDA, IUBMB)
EC Number:
232-808-6
MDL number:
MFCD00071052
UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

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form

lyophilized powder

Quality Level

200

specific activity

≥200,000 units/g protein

mol wt

~300 kDa

composition

protein, ≥40% biuret

storage temp.

−20°C

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This Item
C9281C3766228180
Cholesterol Esterase from Pseudomonas sp. lyophilized powder, ≥200,000 units/g protein

Sigma-Aldrich

C1403

Cholesterol Esterase from Pseudomonas sp.

Cholesterol Esterase from Pseudomonas fluorescens lyophilized powder, ≥10,000 units/g protein

Sigma-Aldrich

C9281

Cholesterol Esterase from Pseudomonas fluorescens

Cholesterol Esterase from bovine pancreas lyophilized powder, ≥200 units/g protein

Sigma-Aldrich

C3766

Cholesterol Esterase from bovine pancreas

Cholesterol Esterase, Microbial Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Sigma-Aldrich

228180

Cholesterol Esterase, Microbial

specific activity

≥200,000 units/g protein

specific activity

≥10,000 units/g protein

specific activity

≥200 units/g protein

specific activity

≥100 units/mg protein, ≥70 units/mg dry wt.

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

~300 kDa

mol wt

~129 kDa

mol wt

84 kDa by SDS-PAGE

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

composition

protein, ≥40% biuret

composition

Protein, ~20%

composition

protein, ≥20%

composition

-

General description

Stability: Stable at –20°C for at least one year
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)

Application

This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.[1]

Other Notes

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000451366
0000451366
0000315675
0000264542
SLCM7419

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Pornpen Srisawasdi et al.
Journal of clinical laboratory analysis, 26(6), 420-430 (2012-11-13)
Accurate determination of cholesterol requires complete hydrolysis of cholesteryl esters and must be very fast for the kinetic cholesterol assay. We investigated the properties of cholesterol esterase derived from Pseudomonas fluorescens, Candida cylindracea, bovine pancreas, and porcine pancreas for cholesterol
Kevin R Shreder et al.
Bioorganic & medicinal chemistry letters, 22(17), 5748-5751 (2012-08-11)
KIAA1363 is a serine hydrolase whose activity has been shown to be positively associated with tumor cell invasiveness. Thus, inhibitors of KIAA1363 represent a novel targeted therapy approach towards cancer. AX11890 ((1-bromo-2-naphthyl) N,N-dimethylcarbamate) was identified as a KIAA1363 inhibitor with
Farid Shokry Ataya
Molecules (Basel, Switzerland), 17(9), 10399-10413 (2012-09-01)
Acid lipase belongs to a family of enzymes that is mainly present in lysosomes of different organs and the stomach. It is characterized by its capacity to withstand acidic conditions while maintaining high lipolytic activity. We cloned for the first
David Y Hui et al.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Javier Sánchez-Marco et al.
Biomedicines, 10(3) (2022-03-26)
Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic Txndc5 expression was observed.
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