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C-14070

Sigma-Aldrich

Human Chondrocytes (HCH)

1 million cells per pellet

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biological source

human articular cartilage (knee and hip joints)

packaging

pkg of 1,000,000 cells

morphology

(chondrocytes)

application(s)

cell analysis (DNA, RNA, and protein)

shipped in

dry ice

storage temp.

−20°C

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This Item
C-12710C-12901C-14080
packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 25,000,000 cells

packaging

pkg of 1,000,000 cells

morphology

(chondrocytes)

morphology

(chondrocytes)

morphology

(mononuclear)

morphology

(myocytes)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

application(s)

cell analysis (DNA, RNA, and protein)

application(s)

-

application(s)

-

application(s)

cell analysis (DNA, RNA, and protein)

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Cartilage

Application

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request). PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Physical form

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Subculture Routine

Click here for more information.

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Ambion, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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Articles

Primary Human Chondrocytes and Chondrocyte Growth Media

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

PromoCell® Primary Cell Pellets

Primary cells from PromoCell are available in pelleted form for research applications like gene expression analysis, and cell/protein characterization experiments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service